WDR5抗体

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     Western blot analysis using WDR5 mouse mAb against Hela (1) cell lysate.    

  
  

WDR5 (WD Repeat Domain 5) is a conserved nuclear protein belonging to the WD40-repeat protein family, known for its role in epigenetic regulation. It serves as a core subunit of multiple histone-modifying complexes, including the MLL/COMPASS and SET1 methyltransferase complexes, which catalyze histone H3 lysine 4 methylation (H3K4me), a hallmark of transcriptionally active chromatin. WDR5 facilitates complex assembly by bridging interactions between catalytic subunits (e.g., MLL1) and other regulatory proteins, thereby influencing gene expression, cell differentiation, and developmental processes. Dysregulation of WDR5 has been implicated in cancers, particularly leukemias, due to its role in maintaining oncogenic gene programs.

WDR5 antibodies are essential tools for studying its function, localization, and interactions. They are widely used in techniques like Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence (IF) to detect WDR5 expression levels, binding partners, and chromatin occupancy. High-quality WDR5 antibodies exhibit specificity for distinct epitopes, enabling researchers to differentiate between isoforms or post-translationally modified forms. Validation methods often include knockout cell lines or competitive peptide blocking to confirm target specificity. Challenges in antibody development arise from WDR5’s structural conservation across species and its participation in diverse multiprotein complexes. Reliable WDR5 antibodies are critical for advancing research in epigenetics, cancer biology, and targeted therapies aiming to disrupt WDR5-dependent oncogenic pathways.