GAPDHS抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: A549 cell lysate, Primary antibody: P04645(GAPDHS Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 2 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using P04645(GAPDHS Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

The GAPDHS antibody targets a unique isoform of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), specifically expressed in spermatogenic cells during mammalian spermatogenesis. Unlike the ubiquitously expressed GAPDH involved in glycolysis, GAPDHS (Glyceraldehyde-3-phosphate dehydrogenase, spermatogenic) is testis-specific and plays a critical role in sperm metabolism, motility, and fertilization. Its expression is tightly regulated during spermatogenesis, peaking in late-stage germ cells and mature spermatozoa.

GAPDHS antibodies are essential tools for studying male fertility, testicular function, and sperm development. They enable the detection and localization of GAPDHS protein in testicular tissues or sperm samples using techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Research using these antibodies has highlighted GAPDHS's role in maintaining energy homeostasis in sperm, particularly in the flagellum, where it supports ATP production for motility.

Dysregulation or mutations in GAPDHS are linked to asthenozoospermia (reduced sperm motility) and male infertility, making it a potential biomarker for diagnostic or therapeutic applications. Additionally, GAPDHS antibodies aid in exploring metabolic adaptations in sperm and evaluating environmental or genetic factors affecting reproductive health. Their specificity ensures minimal cross-reactivity with somatic GAPDH, enhancing reliability in reproductive biology studies.