PITX3抗体

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-3: 231 cell, mouse brain tissue and A549 cell, Primary antibody: P04613(PITX3 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P04613(PITX3 Antibody) at dilution 1/15. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human brain cancer tissue using P04613(PITX3 Antibody) at dilution 1/15. (Original magnification: ×200)    

  
  

The PITX3 antibody is a crucial tool in studying the role of the PITX3 (Paired-Like Homeodomain Transcription Factor 3) protein, a transcription factor essential for embryonic development, particularly in eye lens formation and midbrain dopaminergic neuron differentiation. PITX3. a member of the PITX homeobox gene family, regulates gene expression by binding to specific DNA sequences, influencing cellular differentiation and survival. Its dysfunction is linked to congenital eye disorders (e.g., cataracts, anterior segment dysgenesis) and neurodegenerative conditions like Parkinson’s disease due to dopaminergic neuron loss.

Researchers use PITX3 antibodies primarily in immunohistochemistry (IHC), immunofluorescence (IF), and Western blot (WB) to detect PITX3 expression patterns in tissues (e.g., lens, brain, skeletal muscle) and cell models. These antibodies, often raised in rabbits or mice, are validated for specificity through knockout controls or siRNA knockdown. Commercial variants may differ in clonality (monoclonal/polyclonal), epitope targets, and species reactivity (human, mouse, rat).

PITX3 antibodies also aid in studying developmental biology, stem cell differentiation into dopaminergic neurons, and disease mechanisms. For instance, they help assess PITX3’s role in maintaining midbrain neuron populations or its dysregulation in cataract models. Quality validation (e.g., lot consistency, cross-reactivity checks) is critical to ensure reproducibility, especially given PITX3’s structural homology with other PITX family members.