ARFRP1抗体

+ +

•  

   

     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-4: 293T cells, human fetal liver tissue, human cervical cancer tissue, Human fetal muscle tissue, Primary antibody: P04593(ARFRP1 Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P04593(ARFRP1 Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P04593(ARFRP1 Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

The ARFRP1 (ADP-ribosylation factor-related protein 1) antibody is a tool used to detect and study the ARFRP1 protein, a member of the ARF (ADP-ribosylation factor) family of small GTPases. ARFRP1 plays a regulatory role in intracellular membrane trafficking, particularly in Golgi-to-plasma membrane transport and the organization of the trans-Golgi network. It interacts with other ARF proteins and effectors to modulate vesicle formation, cargo sorting, and organelle positioning. Dysregulation of ARFRP1 has been linked to metabolic disorders, cancer progression, and neurodegenerative diseases, highlighting its importance in cellular homeostasis.

The ARFRP1 antibody is commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression, localization, and post-translational modifications in various tissues and cell lines. Researchers employ it to investigate ARFRP1's role in secretory pathways, lipid metabolism, and cell signaling. Commercial antibodies are typically raised in rabbits or mice, targeting specific epitopes within the conserved GTPase domain or variable regions of the protein. Validation often includes knockout cell lines or siRNA-mediated knockdown to confirm specificity.

Recent studies using ARFRP1 antibodies have uncovered its involvement in insulin granule secretion in pancreatic β-cells and lipid droplet formation in adipocytes, suggesting therapeutic potential for metabolic syndromes. Its expression patterns in tumors, such as breast and colorectal cancers, also make it a biomarker of interest in oncology research.