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MAP2K1抗体

Rabbit Polyclonal MAP2K1 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期：2025-05-09

湖北瑞和宁生物科技有限公司

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产品详情:

中文名称：
MAP2K1抗体
英文名称：
Rabbit Polyclonal MAP2K1 Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 199 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Rabbit
偶联物：
无
靶点：
MAP2K1

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/25-1/100 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/1000-1/5000 Human,Mouse,Rat
   

产品详情

AliasesG3PD; GAPD; MGC88685;Glyceraldehyde-3-phosphate dehydrogenase
Entrez GeneID2597
clone1A10
WB Predicted band size37kDa
Host/IsotypeMouse IgG1
Antibody TypeLoading Control Antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat,Rabbit,Monkey
ImmunogenPurified recombinant fragment of human GAPDH expressed in E. Coli.  
FormulationPurified antibody in PBS with 0.05% sodium azide.

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       Western blot analysis using GAPDH mouse mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate.    


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       Confocal Immunofluorescence analysis of methanol-fixed HepG2 (left) and Hela (right) cells using GAPDH mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.    


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       Immunofluorescence analysis of NIH3T3 cells using GAPDH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.    


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       Flow cytometric analysis of NIH3T3 cells using GAPDH mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of COS7 cells using GAPDH mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using GAPDH mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Mouse colon(A) Mouse pancreas(B) using GAPDH mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Rat stomach using GAPDH mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Rabbit pancreas(A) Rabbit prostate(B) using GAPDH mouse mAb with DAB staining.    


           

参考文献

以下是关于MAP2K1抗体的3篇参考文献及其摘要概括：

1. **文献名称**: *"MEK1 mutations confer resistance to MEK and B-Raf inhibition"*

**作者**: Emery CM, et al.

**摘要**: 该研究利用MAP2K1(MEK1)特异性抗体，通过免疫印迹和免疫组化分析，揭示了MEK1突变在黑色素瘤患者中对靶向治疗的耐药机制，证实抗体在检测突变蛋白表达及定位中的可靠性。

2. **文献名称**: *"Antibody-based profiling of oncogenic signaling pathways in colorectal cancer"*

**作者**: Yuan TL, et al.

**摘要**: 文章系统评估了多种激酶抗体(包括MAP2K1)在结直肠癌组织中的特异性，通过质谱验证抗体结合位点，证明MAP2K1抗体可用于肿瘤样本中MAPK通路活化的临床病理分析。

3. **文献名称**: *"Optimization of phospho-specific antibodies for targeted MAPK pathway analysis"*

**作者**: Pratilas CA, et al.

**摘要**: 研究比较了不同MAP2K1磷酸化抗体(如p-MEK1 Ser217/221)在细胞系和小鼠模型中的检测效能，为癌症研究中抗体选择及信号通路动态监测提供了实验依据。

注：以上内容为示例，实际文献可能需要通过数据库(如PubMed)检索确认。建议使用关键词“MAP2K1 antibody”、“MEK1 antibody application”结合研究领域(如癌症、信号转导)进一步筛选。

       

背景信息

MAP2K1. also known as MEK1. is a dual-specificity kinase within the MAPK/ERK signaling pathway, playing a critical role in regulating cell proliferation, differentiation, and survival. It activates downstream ERK1/2 through phosphorylation of specific tyrosine and threonine residues. MAP2K1 antibodies are essential tools for studying this pathway's activity and dysregulation in diseases like cancer, where mutations (e.g., Q56P, K57N) drive uncontrolled cell growth. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect MAP2K1 expression, phosphorylation status, or mutation-specific variants. Researchers often pair them with phospho-specific ERK antibodies to assess pathway activation. Commercially available MAP2K1 antibodies typically target epitopes within the N-terminal regulatory domain or the C-terminal kinase domain (UniProt: Q02750). Validation includes testing in knockout cell lines or tissues to confirm specificity. In clinical research, MAP2K1 antibodies help identify patients with MEK-inhibitor-sensitive mutations (e.g., in melanoma or NSCLC) and monitor therapeutic responses. Proper controls, such as phosphorylation status verification or mutation-specific blocking peptides, are crucial for experimental accuracy due to pathway cross-talk and homologous sequences in MEK2.
       
MAP2K1抗体;MAP2K1;MAP2K1 Antibody;

公司简介

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成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

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