MDC1抗体

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     The image is immunohistochemistry of paraffin-embedded Human brain tissue using P04377(MDC1 Antibody) at dilution 1/80. (Original magnification: ×200)    

  
  

MDC1 (Mediator of DNA Damage Checkpoint 1) antibody is a key tool for studying the DNA damage response (DDR) pathway. MDC1 is a scaffold protein critical for maintaining genomic stability by facilitating the repair of DNA double-strand breaks (DSBs). It acts as a central coordinator in DDR, interacting with phosphorylated histone H2AX (γ-H2AX) at damage sites and recruiting downstream repair factors like ATM, BRCA1. and 53BP1. MDC1 contains tandem BRCT domains that recognize phospho-epitopes, enabling its role in amplifying DDR signals and activating cell cycle checkpoints.

MDC1 antibodies are widely used in research to investigate DNA repair mechanisms, cancer biology, and cellular responses to genotoxic stress. They are employed in techniques such as Western blotting, immunofluorescence, and co-immunoprecipitation to detect MDC1 expression, localization, and protein interactions. Dysregulation of MDC1 is linked to genomic instability, carcinogenesis, and chemotherapy resistance, making it a potential biomarker or therapeutic target. Commercial MDC1 antibodies are typically raised against specific epitopes (e.g., human MDC1 N-terminal or C-terminal regions) and validated for specificity using knockout cell lines. Researchers often utilize these antibodies to explore MDC1's role in diseases like breast cancer, glioblastoma, and in contexts of radiation or cisplatin-induced DNA damage.