SMG8抗体

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     The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using P04323(SMG8 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P04323(SMG8 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

SMG8 antibody is a research tool designed to study SMG8. a protein component of the nonsense-mediated mRNA decay (NMD) pathway. SMG8. along with SMG7 and SMG9. forms part of the SMG protein family involved in mRNA surveillance. NMD is a conserved eukaryotic mechanism that degrades transcripts harboring premature termination codons (PTCs) to prevent the production of truncated, potentially harmful proteins. SMG8 interacts with SMG1. a phosphatidylinositol 3-kinase-related kinase (PIKK), within the SURF (SMG1-Upf1-eRF1-eRF3) complex, which detects PTCs. Upon recognition of aberrant mRNAs, SMG8 facilitates the transition from mRNA surveillance to degradation by recruiting downstream effectors. Structurally, SMG8 contains a PIN (PilT N-terminal) domain critical for its interaction with SMG1 and regulatory functions.

Antibodies against SMG8 are primarily used in molecular biology to investigate NMD mechanisms, protein-protein interactions (e.g., via co-immunoprecipitation), and subcellular localization (e.g., immunofluorescence). They are typically developed in hosts like rabbits or mice, often validated using knockout cell lines or siRNA-mediated SMG8 depletion. Commercial SMG8 antibodies are commonly applied in Western blotting, with expected bands around 110-130 kDa depending on isoform and post-translational modifications. Dysregulation of SMG8 and NMD has been implicated in cancers and genetic disorders, making these antibodies valuable for studying disease mechanisms and therapeutic targets. Researchers should verify antibody specificity for their experimental models due to potential cross-reactivity with homologous proteins.