Rabbit Polyclonal PRKD1 Antibody

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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: 293T cells, NIH/3T3 cells, Primary antibody: P04305(PRKD1 Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P04305(PRKD1 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P04305(PRKD1 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

The PRKD1 (Protein Kinase D1) antibody is a crucial tool for studying the function and regulation of PRKD1. a serine/threonine kinase belonging to the Ca²+/calmodulin-dependent protein kinase (CAMK) superfamily. PRKD1 plays a pivotal role in diverse cellular processes, including proliferation, apoptosis, migration, and vesicle trafficking. It is activated by various stimuli, such as G protein-coupled receptors (GPCRs) and growth factors, via phosphorylation cascades involving PKC isoforms. Dysregulation of PRKD1 has been implicated in pathological conditions like cancer, cardiovascular diseases, and neurodegenerative disorders, making it a target for therapeutic research.

PRKD1 antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to detect PRKD1 expression, localization, and activation states. These antibodies often target specific epitopes, such as phosphorylated residues (e.g., Ser744/748) critical for kinase activation or conserved regions in the catalytic domain. Validation of PRKD1 antibodies includes testing for specificity using knockout cell lines or siRNA-mediated knockdown. Researchers rely on these antibodies to explore PRKD1's role in signaling pathways (e.g., NF-κB, MAPK) and its crosstalk with oncogenic or tumor-suppressive networks. Commercial availability from multiple vendors, along with varied clonal types (monoclonal/polyoclonal), enhances accessibility for both basic and translational studies.