Rabbit Polyclonal ABCD4 Antibody

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     Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: Jurkat cells, Primary antibody: P04246(ABCD4 Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

**Background of ABCD4 Antibody**

ABCD4. a member of the ATP-binding cassette (ABC) transporter family, plays a critical role in intracellular trafficking and metabolic processes, particularly in vitamin B12 (cobalamin) metabolism. Located on the lysosomal membrane, ABCD4 interacts with lysosomal escort protein LMBRD1 to facilitate the transport of vitamin B12 from lysosomes into the cytoplasm, a vital step for its conversion into active cofactors required for mitochondrial enzyme function. Dysregulation of ABCD4 is linked to hereditary disorders of cobalamin metabolism, such as the cblJ-type combined homocystinuria and methylmalonic aciduria.

ABCD4 antibodies are essential tools for studying the protein’s expression, localization, and functional interactions. Structurally, ABCD4 contains a transmembrane domain and a nucleotide-binding domain (NBD) that hydrolyzes ATP to drive substrate transport. Researchers utilize ABCD4-specific antibodies in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate its role in cellular homeostasis, disease mechanisms, and potential therapeutic targets. These antibodies also aid in diagnosing genetic disorders by detecting ABCD4 mutations or aberrant expression in patient samples. Recent studies highlight ABCD4’s broader implications in lysosomal function and metabolic diseases, underscoring its significance beyond cobalamin metabolism. As research advances, ABCD4 antibodies remain pivotal in unraveling the protein’s molecular mechanisms and clinical relevance.