Rabbit Polyclonal SMAD7 Antibody

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: LO2 cell lysate, Primary antibody: P04203(SMAD7 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 minute    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P04203(SMAD7 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

SMAD7. a member of the inhibitory SMAD (I-SMAD) family, plays a critical role in regulating transforming growth factor-beta (TGF-β) signaling pathways. As a key negative feedback regulator, SMAD7 binds to activated TGF-β or bone morphogenetic protein (BMP) type I receptors, blocking the phosphorylation and activation of receptor-regulated SMADs (R-SMADs, e.g., SMAD2/3 or SMAD1/5/8). This interaction promotes receptor ubiquitination and degradation, effectively dampening downstream signaling. Dysregulation of SMAD7 has been implicated in various pathological conditions, including fibrosis, cancer, and inflammatory diseases, where its expression often correlates with disease progression or therapeutic resistance.

SMAD7 antibodies are essential tools for investigating these biological processes. They enable the detection and quantification of SMAD7 protein levels in experimental models and clinical samples through techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers utilize these antibodies to study SMAD7's subcellular localization, protein-protein interactions, and post-translational modifications. Commercially available SMAD7 antibodies are typically developed in host species such as rabbits or mice, targeting specific epitopes within the protein's N-terminal or linker regions. Validation parameters include specificity checks via knockout controls and functional assays. Applications span mechanistic studies in TGF-β pathway modulation, drug development screening, and biomarker analysis in diseases like colorectal cancer, pancreatitis, and autoimmune disorders.