Rabbit Polyclonal RAD50 Antibody

The RAD50 antibody is a crucial tool in studying the RAD50 protein, a key component of the MRE11-RAD50-NBS1 (MRN) complex involved in DNA damage response and repair. RAD50 plays a central role in detecting and repairing DNA double-strand breaks (DSBs), maintaining genomic stability through homologous recombination repair, and regulating cell cycle checkpoints. Structurally, RAD50 contains ATP-binding and ATPase domains critical for its DNA-binding and nuclease activation functions. Antibodies targeting RAD50 are widely used in research to investigate its expression, localization, and interactions via techniques like Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF). Dysregulation or mutations in RAD50 are linked to genomic instability disorders, cancer predisposition, and radiation sensitivity. RAD50 antibodies also aid in diagnosing conditions like Nijmegen breakage syndrome and studying therapeutic responses in oncology. These antibodies often target specific epitopes, such as the coiled-coil or ATPase domains, enabling functional studies of RAD50's role in DNA repair pathways. Their application extends to exploring correlations between RAD50 expression levels and clinical outcomes in cancers, including breast and ovarian malignancies.