Rabbit Polyclonal RAD52 Antibody

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: K562, PC3 and SKOV3 cell lysates, Primary antibody: P04188(RAD52 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 30 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P04188(RAD52 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P04188(RAD52 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The RAD52 antibody is a crucial tool in studying the RAD52 protein, a key player in DNA repair and genome maintenance. RAD52 facilitates homologous recombination (HR) and single-strand annealing (SSA), critical pathways for repairing DNA double-strand breaks (DSBs). It binds single-stranded DNA (ssDNA), promoting strand pairing and exchange during HR, and stabilizes DNA intermediates. In yeast, RAD52 is essential for HR, but in mammals, its role is partially supplanted by BRCA1/2 proteins, though it remains vital in backup repair mechanisms, particularly in BRCA-deficient cancers.

Researchers use RAD52 antibodies to detect protein expression, localization, and interactions in various experimental models, including human, mouse, and yeast cells. These antibodies are employed in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate RAD52's dynamics during DNA damage responses. Elevated RAD52 levels are observed in certain cancers, linking it to genomic instability and therapeutic resistance. Inhibiting RAD52 is explored as a strategy to target BRCA-mutant tumors, exploiting synthetic lethality.

The antibody's specificity and validation (e.g., knockout controls) are critical for accurate data interpretation. Studying RAD52 also sheds light on replication stress, telomere maintenance, and viral integration mechanisms. Overall, RAD52 antibodies are indispensable for unraveling DNA repair pathways and their implications in cancer biology and treatment.