Rabbit Polyclonal PAX1 Antibody

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     Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: K562 cells, Primary antibody: P04166(PAX1 Antibody) at dilution 1/1200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes    

  
  

The PAX1 antibody is a tool used to detect the PAX1 (Paired Box 1) protein, a member of the PAX family of transcription factors critical in embryonic development. PAX1 contains a paired box domain that binds DNA, regulating gene expression involved in tissue differentiation, particularly in skeletal and thymic development. It plays a key role in patterning the vertebral column, thymus, and parathyroid glands by directing mesenchymal cell differentiation. Dysregulation of PAX1 is linked to developmental disorders, such as vertebral malformations (e.g., spondylocostal dysostosis) and thymic hypoplasia, as well as cancers like chordoma or squamous cell carcinoma.

PAX1 antibodies are widely utilized in research to study its expression and function via techniques like immunohistochemistry, Western blotting, and immunofluorescence. These antibodies help map PAX1’s spatial-temporal expression during development and assess its aberrations in diseases. Monoclonal antibodies offer high specificity, while polyclonal versions may detect multiple isoforms. Validation often involves knockout controls or peptide blocking to confirm target specificity.

Recent studies explore PAX1’s potential as a biomarker for cancer prognosis or as a therapeutic target. Its role in epithelial-mesenchymal interactions and stem cell regulation also makes it relevant in regenerative medicine. However, challenges remain in standardizing antibody performance across experimental models. Overall, PAX1 antibodies remain vital for unraveling developmental pathways and disease mechanisms.