Rabbit Polyclonal PIF1 Antibody

PIF1 antibodies are essential tools for studying the PIF1 helicase, a conserved DNA helicase crucial for maintaining genomic stability. PIF1 (PDB: Pfam) is involved in diverse DNA metabolic processes, including replication fork restart, repair of stalled replication forks, telomere length regulation, and mitochondrial DNA maintenance. In humans, PIF1 exists as two isoforms: a nuclear form (hPIF1) and a mitochondrial-targeted variant (hPIF1β), each with distinct roles. Yeast Pif1. a well-characterized homolog, facilitates Okazaki fragment maturation, suppresses telomerase activity, and resolves G-quadruplex structures. Antibodies against PIF1 enable researchers to detect its expression, localization, and interaction partners through techniques like Western blotting, immunofluorescence, and immunoprecipitation. These reagents are critical for investigating PIF1's involvement in diseases, particularly cancers linked to replication stress (e.g., BRCA-deficient tumors) and age-related disorders associated with mitochondrial dysfunction. Commercially available PIF1 antibodies are typically raised in rabbits or mice, using immunogens derived from conserved regions of the protein. Validation often includes testing in PIF1-knockout cell lines or recombinant protein systems. As dysregulation of PIF1 is implicated in genomic instability and tumorigenesis, its antibodies also support therapeutic research targeting helicase pathways. Cross-reactivity with PIF1 orthologs (e.g., mouse, yeast) further broadens their utility in comparative studies.