Rabbit Polyclonal HINT3 Antibody

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: HepG2 cell, Human liver tissue lysates, Primary antibody: P04021(HINT3 Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 minute    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P04021(HINT3 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

**Background of HINT3 Antibody**

The HINT3 (Histidine Triad Nucleotide-Binding Protein 3) antibody is a tool used to study the HINT3 protein, a member of the histidine triad (HIT) superfamily. HINT3. encoded by the *HINT3* gene, is characterized by a conserved histidine triad motif (HφHφHφφ; φ = hydrophobic residue) that facilitates nucleotide binding and hydrolysis. Unlike other HIT proteins, such as HINT1 or HINT2. HINT3 remains less characterized, though it is hypothesized to play roles in nucleotide metabolism, cellular signaling, or tumor suppression.

HINT3 is ubiquitously expressed, with higher levels observed in tissues like the liver, kidney, and brain. Its subcellular localization varies, potentially linked to interactions with proteins involved in transcriptional regulation or stress responses. Studies suggest HINT3 may influence apoptosis, DNA repair, and redox homeostasis, though mechanistic insights remain limited.

The HINT3 antibody enables the detection and quantification of HINT3 in experimental models, aiding research into its physiological and pathological functions. It is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Dysregulation of HINT3 has been tentatively associated with cancers, neurodegenerative diseases, and metabolic disorders, making this antibody valuable for exploring disease mechanisms.

Despite progress, HINT3's precise biological roles and substrates require further elucidation. The development and validation of specific HINT3 antibodies continue to support ongoing investigations into its molecular interactions and therapeutic potential.