This certificate is designed in accordance with ISO 17034 and
ISO Guide 31. This reference material
(RM) was designed,produced and verified in accordance with ISO/IEC
17025, ISO 17034 and a registered
quality management system ISO 9001.
Product Name(产品名称)10 Whitening and Freckle-removing Mix in Methanol and Water(1:24),100μg/mL(甲醇和水中10种美白祛斑剂混标(1:24))
Transportation(运输条件) Normal temperature transportation
(常温运输)
CERTIFIED
Component No. (组分数)
Peak Sequence (出峰顺序)
Component (组分)
CAS No.
Concentration (μg/mL) (浓度)
Relative Expanded Uncertainty(%)
(k=2) (相对扩展不确定度)
1
1
Magnesium Ascorbyl Phosphate2(维生素C磷酸酯镁)
113170-55-1
100
3
2
2
Ascorbyl Glucoside2(抗坏血酸葡糖苷)
129499-78-1
100
3
3
3
Arbutin1(熊果苷)
497-76-7
100
3
4
4
Kojic Acid2(曲酸)
501-30-4
100
3
5
5
Nicotinamide1(烟酰胺)
98-92-0
100
3
6
6
3-O-Ethyl-L-ascorbic Acid2(3-O-乙基抗坏血酸醚)
86404-04-8
100
3
7
7
Potassium Methoxysalicylate2(甲氧基水杨酸钾)
152312-71-5
100
3
8
8
Raspberry Ketone Glucoside1(覆盆子酮葡糖苷)
38963-94-9
100
3
9
9
Dipotassium Glycyrrhizinate2(甘草酸二钾)
68797-35-3
100
3
10
10
4-Butylresorcinol1(4-正丁基间苯二酚)
18979-61-8
100
3
Instrument(检测仪器) HPLC/DAD
Column(色谱柱) C18(3.0x100mm,1.8μm)
Column Oven(柱温) 35℃
Wavelength(波长) 230nm,250nm
Method Details(方法参数) A: Methanol(甲醇) B: 0.02mol/L Potassium Phosphate Monobasic,Adjust the PH with potassium hydroxide,PH=6.0 (0.02mol/L磷酸二氢钾，用氢氧化钾调节PH值，PH=6.0)
Time/min
A/%
B/%
0.00
5
95
3.00
5
95
8.00
20
80
14.00
90
10
18.00
90
10
23.00
5
95
Note
1. Arbutin(CAS No. : 497-76-7),Nicotinamide(CAS No. : 98-92-0),Raspberry Ketone Glucoside(CAS No. : 38963-94-9) and 4-Butylresorcinol(CAS No. : 18979-61-8) are calculated by the peak area at 230nm.(熊果苷(CAS No. : 497-76-7)、烟酰胺(CAS No. : 98-92-0)、覆盆子酮葡糖苷(CAS No. : 38963-94-9)和4-正丁基间苯二酚(CAS No. : 18979-61-8)是根据230nm波长处的峰面积计算的。) 2.Magnesium Ascorbyl Phosphate(CAS No. : 113170-55-1),Ascorbyl Glucoside(CAS No. : 129499-78-1),Kojic Acid(CAS No. : 501-30-4),3-O-Ethyl-L-ascorbic Acid(CAS No. : 86404-04-8) ,Potassium Methoxysalicylate (CAS No. : 152312-71-5) and Dipotassium Glycyrrhizinate(CAS No. : 68797-35-3) are calculated by the peak area at 250nm.(维生素C磷酸酯镁(CAS No. : 113170-55-1)、抗坏血酸葡糖苷(CAS No. : 129499-78-1)、曲酸(CAS No. : 501-30-4)、3-O-乙基抗坏血酸醚(CAS No. : 86404-04-8)、甲氧基水杨酸钾(CAS No. : 152312-71-5)和甘草酸二钾(CAS No. : 68797-35-3) 根据250nm波长处的峰面积计算的。)
Intended Use
This RM is intended for use in a laboratory as a calibration and quality control standard or in
method development for analytical techniques.
Uncertainty
The certified value(s) and uncertainty(ies) are determined in accordance with ISO
17034 with an 95%
confidence level (k=2). Uncertainty is based on the Total Combined Uncertainty, including
uncertainties of preparation, purity of neat materials, homogeneity, stability testing.
Traceability
The balances used for gravimetric measurements are calibrated with weights traceable to the national
standards. The calibration of the balances is verified annually by an external accredited
calibration service. This analysis method has been verified using an approach consistent with
ISO 17034:2016 & ISO 17025:2017.
Homogeneity
Random replicate samples of the final packaged RM have been analysed to prove homogeneity consistent
with ISO 17034.
Storage
The RM should be stored in the original sealed bottle at the indicated temperature.
