重组人PSME3蛋白-ACROBiosystems百普赛斯

Human PSME3 / PA28g Protein, His Tag

分子别名（Synonym）

PSME3,PA28gamma,REG-gamma

表达区间及表达系统（Source）

Human PSME3, His Tag (PS3-H5142) is expressed from E. coli cells. It contains AA Ala 2 - Tyr 254 (Accession # P61289-1).

Predicted N-terminus: Met

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 30.3 kDa. The protein migrates as 28-33 kDa under reducing (R) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

蛋白酶体激活物复合物亚基3（PSME3）是PA28家族的成员，也称为11S调节物复合物γ亚基（REGγ）、多催化蛋白酶亚基3的激活剂和蛋白酶体激活剂28亚基γ（PA28γ）。PSME3激活蛋白酶体的胰蛋白酶样催化亚基，但抑制胰凝乳蛋白酶样和后谷氨酰偏好（PGPH）亚基。PSME3还可以促进MDM2-p53/TP53相互作用，促进p53/TP53的泛素化和MDM2依赖性蛋白酶体降解，限制其积累，导致DNA损伤后细胞凋亡受到抑制。此外，PSME3也可能参与细胞周期调控。