重组人BTN3A2蛋白-ACROBiosystems百普赛斯

Biotinylated Human BTN3A2 Protein, His,Avitag™

分子别名（Synonym）

BTN3A2,BT3.2

表达区间及表达系统（Source）

Biotinylated Human BTN3A2, His,Avitag (BT2-H82E7) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Trp 248 (Accession # P78410-1).

Predicted N-terminus: Gln30

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 27.3 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Biotinylation）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

肿瘤细胞表达的亲丁蛋白亚家族3成员A2（BTN3A2/BT3.2）mRNA先前被鉴定为高级别浆液性上皮性卵巢癌症（HG-EOC）小队列中的预后因素。我们发现三种BTN3A亚型：BTN3A1、BTN3A2和BTN3A3与B7超家族的蛋白质具有高度的结构同源性，在溶液中以V形同源二聚体存在，通过膜近端C型Ig结构域结合。