EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™
CC-3162
Culture system containing EBMTM-2 Basal Medium (CC-3156) and EGMTM-2 SingleQuotsTM Supplements (CC-4176) required
for growth of Endothelial Cells. Available for immediate shipment.
EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™
Catalog #: CC-3162
Culture system containing EBMTM-2 Basal Medium (CC-3156) and EGMTM-2 SingleQuotsTM Supplements (CC-4176) required
for growth of Endothelial Cells. Available for immediate shipment.
Request Information or Quote or alternatively, generate the quote straight away by adding the product to the cart and simply
clicking on “Generate Quote”.
Do you need a different format or volume for this media product? Contact Lonza CellBio Services to discuss custom format and
formulation options.
Select Size
6 KITS
1 KIT
FGM™-2 Fibroblast Growth Medium-2 BulletKit™
CC-3132
Culture system contains FBMTM Basal Medium (CC-3131) and FGMTM-2 SingleQuotsTM supplements (CC-4126) required for
growth of fibroblasts.
Product Overview
FGMTM-2 Fibroblast Growth Medium-2 was developed to support the growth of most primary human fibroblasts including
lung and dermal fibroblasts. The kit contains basal medium and supplements packaged together for convenience to culture
fibroblasts. FGMTM-2 Medium contains 2% serum.
Purchase our Lung Fibroblast Cell Culture Kit (catalog no. CC-2512B) and get a discounted price on cells and media. The kit
consists of a cryopreserved ampule of lung fibroblast cells (catalog no. CC-2512) and FGMTM-2 BulletKitTM Growth Media
(catalog no. CC-3132)
Storage and Content
1 x FBMTM Basal Medium (CC-3131), 500 mL
1 x FGMTM-2 SingleQuotsTM Supplement Pack (CC-4126) containing:
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Gray Cap Vial with hFGF-B, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Bottle FBS, 10.00 mL
HCMTM Hepatocyte Culture Medium BulletKitTM
CC-3198
Hepatocyte Culture Medium containing HBMTM Basal Medium (CC-3199) and HCMTM SingleQuot Supplements (CC-4182)
required for growth of primary hepatocytes.
Product Overview
The Hepatocyte Culture Medium BulletKitTM consists of the HBMTM Hepatocyte Basal Medium, 500 mL (CC-3199) and the
HCMTM SingleQuotsTM Kit (CC-4182).
Once combinded together these reagents formulate a highly reproducible maintenance medium for primary hepatocytes from
all species. The final medium is serum-free.
Storage and Content
1 x HBMTM (CC-3199) Bottle Basal Medium, 500 mL
1 x HCMTM (CC-4182) SinglequotsTM Supplement Pack containing:
1 x Transferrin, 0.50 mL
1 x Ascorbic Acid, 0.50 mL
1 x HEGF, 0.50 mL
1 x Insulin, 0.50 mL
1 x Hydrocortisone, 0.50 mL
1 x BSA (Fatty Acid Free), 10.5 mL
1 x GA-1000, 0.50 mL
REGM™ Renal Epithelial Cell Growth Medium BulletKit™
CC-3190
Culture system containing REBMTM Basal Medium (CC-3191) and REGMTM SingleQuotsTM supplements (CC-4127) required
for growth of Renal Epithelial Cells.
Product Overview
REGMTM Renal Epithelial Cell Growth Medium is formulated to maintain and expand most renal epithelial cells. REGMTM
contains 0.5% serum.
Select References:
Diabetic Nephropathy is a chronic, progressive renal disease/diabetic kidney disease that develops in approximately one third
of people with diabetes. In a study, Lonza’s human proximal tubular epithelial cells were cultured in respective media
(REGMTM). The group investigated the role of KLK1 (kallikrein) in tubular pro-inflammatory responses in cultured human RPTEC
and examined the role of PAR-4 (Protease-Activated Receptor 4) activation in KLK1-mediated signaling in the development of
DN. Yiu WH, Wong DW, Chan LY, Leung JC, Chan KW, Lan HY, Lai KN, Tang SC. Tissue Kallikrein Mediates Pro-Inflammatory
Pathways and Activation of Protease-Activated Receptor-4 in Proximal Tubular Epithelial Cells. PLoS ONE (2014); 9(2): e88894
Renal Graft Research: In vitro renal epithelial cell culture models have provided a great deal of information about the outcome
of renal clinical transplantation models. In a study carried out using Lonza’s Primary Human Renal Proximal Tubular Epithelial
cells maintained in REGMTMTM renal epithelial growth media (cat# CC-2553 & CC-3190 respectively), the group study the
effects of HCMV (Human cytomegalovirus) infection on renal cells. Masako Shimamura, Joanne E. Murphy-Ullrich and
William J. Britt. Human Cytomegalovirus Induces TGF-β1 Activation in Renal Tubular Epithelial Cells after
Epithelial-to-Mesenchymal Transition. PLoS Pathogens. (2010); 6(11): e1001170
Storage and Content
1 x REBMTM Basal Medium (CC-3191), 500 mL
1 x REGMTM SinglequotsTM Supplement Pack (CC-4127) containing:
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Bottle FBS, 2.50 mL
1 x Natural Cap Vial with Transferrin, 0.50 mL
1 x Amber Vial with Triiodothyronine, 0.50 mL
1 x Amber Vial with Epinephrine, 0.50 mL
BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit™
CC-3170
Culture system containing BEBMTM Bronchial Epithelial Cell Growth Basal Medium (CC-3171) and BEGMTM Bronchial Epithelial
Cell Growth Medium SingleQuotsTM Supplements and Growth Factors (CC-4175).
Co-culture of Asthmatic Bronchial Epithelial and Smooth Muscle Cells in Monolayer
Co-culture of asthmatic bronchial epithelial and smooth muscle cells grown in BEGM™ and SmGM™ 2 BulletKit™ and stained
with anti-alpha-smooth muscle actin
Product Overview
BEGMTM Bronchial Epithelial Cell Growth Medium is specially designed to support the growth of human primary bronchial
epithelial cells. BEGMTM BulletKitTM is serum-free and has been optimized to grow cells from normal and diseased tissues.
Published research shows BEGMTM BulletKitTM has expanded applications. Our customers have used BEGMTM BulletKitTM to
culture well-established cell lines such as BEAS-2B, home-grown bronchial epithelial cells and supporting co-culture models
with breast cancer cell lines such as MCF-7 and MDA-MB-231. Refer to the published literature in the 'Data' tab.
BEGMTM is an optimized and readily available media kit providing researchers the confidence and the convenience of a system
that works to support their cells. This is especially important for researchers who are utilizing cell lines as a stepping stone to
primary cell culture. Working with the same BEGMTM Media between cell lines and primary NHBE can reduce variability within
the experiments and simplify the shift for researchers. In addition, if primary cells are utilized as a side-by-side control with
normal cell lines or home-grown bronchial epithelial cells, the BEGMTMBulletKitTM Media may also offer cost-benefit since
customers can use the same media to support multiple cell types.
Here are publications out of many where our customers have used BEGMTM Growth Media with normal bronchial epithelial cells
El Najjar, et al. (2015). PLoSONE 10(2) demonstrates culturing of BEAS-2B bronchial epithelial cell line in BEGMTM Media to
examine the effects of cathepsin and furin proteolytic enzymes on viral fusion protein activation in cells
Hagiwara, et al. (2015). Nature demonstrates BEGMTM Media can ideally support co-culture of Lonza's HUVEC and NHBE in a
3D environment.
Rider, et al. (2015). PLoS ONE 10(1) used BEGMTM Media to culture primary human bronchial epithelial cells obtained from
non-transplanted normal lungs
Purchase our Bronchial Epithelial Cell Culture Kit (catalog#. CC-2540B) and get a discounted price on cells and media. The kit
consists of a cryopreserved ampule of bronchial epithelial cells (catalog #CC-2540) and BEGMTM BulletKitTM Growth Media
(catalog#. CC-3170).
Storage and Content
1 x BEBMTM Basal Medium (CC-3171), 500 mL
1 x BEGMTM SingleQuotsTM Supplement Pack (CC-4175) containing:
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Amber Vial with Retinoic Acid, 0.50 mL
1 x Natural Cap Vial with Transferrin, 0.50 mL
1 x Amber Vial with Triiodothyronine, 0.50 mL
1 x Amber Vial with Epinephrine, 0.50 mL
1 x Green Cap Vial with hEGF, 0.50 mL
EGMTM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM
CC-3202
Culture system containing EBMTM-2 Basal Medium (CC-3156) and EGMTM-2 MV Microvascular Endothelial Cell Growth Medium SingleQuotsTM
supplements (CC-4147) required for growth of Microvascular Endothelial Cells
EGM® 2MV Microvascular Endothelial Cell Growth Medium-2 BulletKit®
Includes basal medium and SingleQuots® Kit
Product Overview
Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has
its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used
to grow CloneticsTM Primary Cells.
The following Endothelial Cell Growth Medium BulletKitsTM are available:
EGMTM-2 BulletKitTM – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Final concentration of FBS in the mixed
BulletKit is 2%. Contains VEGF for rapid proliferation. Does not contain BBE.
EGMTM BulletKitTM – Optimized for HUVECs cat. no. CC-2517 and CC-2519. Contains BBE .Final concentration of FBS in the mixed BulletKit is
2%. Does not contain exogenous VEGF.
EGMTM-Plus BulletKitTM – Enhanced version of EGMTM Medium. Optimized for HUVECs cat. no. CC-2935. Contains BBE. Final concentration
of FBS in the mixed BulletKit is 2%. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGMTM Medium.
EGMTM-2MV BulletKitTM – Coronary and Iliac artery, human microvascular endothelial cells.
Final concentration of FBS in the mixed BulletKit is 5%. Contains VEGF for rapid proliferation. Does not contain BBE.
Lonza guarantees the performance of CloneticsTM/PoieticsTM Cells only if appropriate CloneticsTM/PoieticsTM Media and Reagents are used
exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including
the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the
appropriate media, reagents, or protocol, please contact Lonza Scientific Support.
Storage and Content
1 x EBMTM-2 Basal Medium (CC-3156), 500 mL
1 x EGMTM-2 MV SingleQuotsTM Supplement Pack (CC-4147) containing:
1 x Bottle FBS, 25.00 mL
1 x Natural Cap Vial with Hydrocortisone, 0.20 mL
1 x Gray Cap Vial with hFGF-B, 2.00 mL
1 x White Cap Vial with VEGF, 0.50 mL
1 x Yellow Cap Vial with R3-IGF-1, 0.50 mL
1 x Blue Cap Vial with Ascorbic Acid, 0.50 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 m
