HyClone Minimal Essential Medium (MEM) variations: Liquid
SH30265.01
500 mL
MEM Alpha modification, with L-glutamine, ribo- and deoxyribonucleosides
HyClone MEM media promotes cell growth in research and biomanufacturing cell culture processes.
Overview
HyClone MEM liquid media supports cell growth in research, upstream process development, and cell culture manufacturing
processes.
Minimum essential medium efficacy and homogeneity is tested with Cytiva HyClone sera.
MEM composition includes vitamins, minerals, and amino acids vital to supporting mammalian cell culture and promoting cell
growth. There are several MEM media options to support cell culture process development. Formulations of HyClone cell
culture MEM media are available with or without L-glutamine. The MEM alpha modification includes the additional components
such as non-essential amino acids, ascorbic acid, biotin, vitamin B12, lipoic acid, sodium pyruvate, ribonucleosides and
deoxyribonucleosides. Improved MEM, or Richter’s modification, is also available with increased concentrations of certain
amino acids, vitamins, and metabolites. In many MEM media formulations, phenol red is added as a pH indicator. All available
MEM media contain a sodium bicarbonate buffer with some also containing EBSS to maintain optimal culture pH.
HyClone MEM raw material components are screened through strict quality control testing for lot-to-lot consistency. MEM
liquid media products are hydrated using purified water and undergo sterile filtration.
HyClone Dulbecco's Modified Eagle Medium (DMEM) with low glucose: Liquid
SH30021.01
500 mL
DMEM/Low glucose with L-glutamine, sodium pyruvate
Overview
Supports cell growth in research and biomanufacturing cell culture processes.
Raw material components screened through strict quality control testing for lot-to-lot consistency.
Product efficacy and homogeneity tested with Cytiva HyClone sera.
Products are hydrated using purified process water and undergo sterile filtration.
HyClone Dulbecco's Modified Eagle Medium (DMEM) with high glucose: Liquid
SH30243.02
1000 mL
DMEM/High glucose with L-glutamine, sodium pyruvate
Overview
HyClone DMEM high glucose liquid media supports cell growth in research, upstream process development, and cell culture
manufacturing processes.
DMEM high glucose medium efficacy and homogeneity is tested with HyClone sera.
DMEM media contain increased concentrations of vitamins, minerals, and amino acids compared to traditional basal media.
DMEM medium composition also includes ferric nitrate, making this cell culture medium an option for certain cell lines requiring
iron for cell growth. HyClone DMEM media preparations are available in high or low glucose formulations, as well as with or
without L-glutamine, magnesium, and sodium pyruvate. DMEM formulation options may also include phenol red as a pH
indicator. All available DMEM media products contain a sodium bicarbonate buffer with or without HEPES to maintain optimal
culture pH.
HyClone DMEM raw material components are screened through strict quality control testing for lot-to-lot consistency. DMEM
liquid media products are hydrated using purified water and undergo sterile filtration.
HyClone RPMI 1640 media: Liquid
SH30809.01
Supports cell growth in research and biomanufacturing cell culture processes.
HyClone Leibovitz L-15 medium: Liquid
SH30525.01
500 mL
Overview
Supports cell growth in research and biomanufacturing cell culture processes.
Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities.
Raw material components screened through strict quality control testing for lot-to-lot consistency.
Products are hydrated using purified process water and undergo sterile filtration.
HyClone SFX-Insect cell culture media
SH30278.02
1000 mL
SFX-Insect liquid medium with L-glutamine, sodium bicarbonate - 1000 mL
Serum-free medium supports recombinant protein production with multiple insect cell lines.
Designed for high cell yield and recombinant protein production.
Complexed lipids for enhanced stability.
Supports direct and sequential adaptation
High performance growth in multiple culture systems including high density bioreactors.
Manufactured using ISO 9001 and ISO 13485 certified processes according to 21 CFR 820 medical device current good
manufacturing practices (cGMP).
SFX-Insect medium provides excellent growth of many key insect cell lines, including Sf9, Sf21, High Five (T.ni) and D.Mel-2 cells,
while requiring minimal adaptation. Additionally, SFX-Insect medium has been successfully tested in a variety of culture systems
ranging from T-flasks, shaker flasks, and various traditional and disposable bioreactors. SFX-Insect medium is available as liquid
or powder medium in bottles, bags, or custom packaging.
HyClone Dulbecco's Modified Eagle Medium (DMEM)/ F12 1:1: Liquid
SH30023.01
500 mL
DMEM/F12 1:1 medium with L-glutamine, HEPES
Overview
Supports cell growth in research and biomanufacturing cell culture processes.
Raw material components screened through strict quality control testing for lot-to-lot consistency.
Product efficacy and homogeneity tested with Cytiva HyClone sera.
Products are hydrated using purified process water and undergo sterile filtration.
