重组人KIR2DL4/CD158d蛋白-ACROBiosystems百普赛斯

Human KIR2DL4 / CD158d Protein, Fc Tag

分子别名（Synonym）

KIR2DL4,CD158d

表达区间及表达系统（Source）

Human KIR2DL4, Fc Tag (KI4-H5255) is expressed from human 293 cells (HEK293). It contains AA His 24 - His 242 (Accession # Q99706-1).

Predicted N-terminus: His 24

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 50.5 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

杀伤细胞免疫球蛋白样受体2DL4（KIR2DL4）属于免疫球蛋白超家族，是NK细胞的抑制性受体。可溶性HLA-G与内体中的KIR2DL4结合，激活DNA-PKcs（DNA依赖性蛋白激酶，催化亚基）-AKT-NF-κB信号通路。KIR2DL4蛋白ITIM的刺激导致非受体蛋白磷酸酶Src同源2结构域酪氨酸磷酸酶（SHP）-1和SHP-2的募集和激活，并随后抑制人NK细胞中的CD16/FcγRIIIa信号通路。