重组人Huntingtin/HTT蛋白-ACROBiosystems百普赛斯

HTT产品信息

英文名称：Huntingtin

中文名称：亨廷顿蛋白

靶点别称：HTT

物种：Human

标签：His Tag & MBP Tag

属性：Protein

标记：Unconjugated

表达区间及表达系统（Source）

Human HTT, MBP,His Tag (HTT-H51M5) is expressed from E. coli cells. It contains AA Met 1 - Glu 82 (Accession # P42858-1).

Predicted N-terminus: Met

蛋白结构（Molecular Characterization）

This protein carries a MBP tag at the N-terminus and a polyhistidine tag at the C-terminus

The protein has a calculated MW of 52.7 kDa. The protein migrates as 60-63 kDa under reducing (R) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

亨廷顿蛋白是由HTT基因编码的蛋白质。亨廷顿蛋白（Htt）中的常染色体显性突变是亨廷顿氏病（HD）的病因。在HD中，蛋白质N端序列中的聚谷氨酰胺（polyQ）结构域扩展到超过36个谷氨酰胺的阈值。突变型polyQ扩增与发病年龄呈负相关。