11-Ketotestosterone ELISA Kits
Arbor Assays 11-酮睾酮 ELISA 试剂盒
K079-H
The DetectX® 11-Ketotestosterone Immunoassay Kit uses a specifically generated antibody to measure 11-ketotestosterone in
urine or in extracted fecal, serum, and plasma samples.
Specifications
Catalog Number
K079-H
Assay Type
Competitive ELISA
Sample Types
Extracted Serum, Extracted Plasma, Urine, Fecal Extracts
Sensitivity
1.85 pg/mL
Species
11-Ketotestosterone is identical across species
Assay Duration
2.5 Hours
Samples/Plate
39 in duplicate
Readout
Colorimetric, 450 nm
Assay Principle:
The 11-Ketotestosterone ELISA Kit quantitatively measures 11-Ketotestosterone in urine, extracted plasma, extracted serum,
and dried fecal extracts. This competitive ELISA has a run time of 2.5 hours. Please read the complete kit insert for more
information before performing this assay. Use our provided 11-Ketotestosterone standard to generate a standard curve for
the assay.
Protocol Summary:
Introduce standards or diluted samples into the transparent microtiter plate, pre-coated with goat anti-rabbit IgG antibody.
Add 11-Ketotestosterone peroxidase conjugate and 11-Ketotestosterone rabbit polyclonal antibody to each well.
Incubate the mixture covered at room temperature with shaking for 2 hours, facilitating the immunological reaction.
After incubation, wash the plate to remove excess conjugate. Add the TMB substrate, which interacts with the bound conjugate
to produce a detectable signal.
Use a plate reader to detect the signal at 450nm and calculate the 11-Ketotestosterone concentration using the standard curve.
Background:
Androgenic hormones like testosterone regulate growth, size, and reproduction in many male organisms. In teleost fish,
11-ketotestosterone, alongside testosterone, plays a significant role in inducing reproductive characteristics in both genders.
Particularly in male fish, 11-ketotestosterone levels surge during spermatogenesis in the spawning season. In females, this
hormone regulates ovarian development by increasing before yolk deposition.
The role of 11-ketotestosterone extends beyond aquatic species. Recent research has established its presence and function in
humans and other mammals. Unlike in fish, primate serum 11-ketotestosterone concentrations show no significant gender-base
d differences, indicating a non-testis-dependent production, likely originating from adrenal-derived 11-oxyandrogen precursors.
Interestingly, higher levels of 11-ketotestosterone have been found in prepubertal children and postmenopausal women than in
men. This finding suggests it could be used as a clinical biomarker for adrenal androgen excess in conditions like polycystic
ovary syndrome (PCOS) and hirsutism in women.
The DetectX® 11-Ketotestosterone ELISA Kit is an essential tool for endocrinologists and researchers, enabling the precise
measurement of 11-Ketotestosterone levels in various biological samples. This kit facilitates the study of its physiological roles
and potential clinical applications in human health and disease.
