重组人GUCY2D蛋白-ACROBiosystems百普赛斯

Biotinylated Human GUCY2D / Retinal guanylyl cyclase 1 Protein, His,Avitag™

分子别名（Synonym）

GUCY2D,Retinal guanylyl cyclase 1,RETGC-1,CORD6,GUC1A4,GUC2D,ROS-GC

表达区间及表达系统（Source）

Biotinylated Human GUCY2D, His,Avitag (GUD-H82E6) is expressed from human 293 cells (HEK293). It contains AA Ala 52 - Glu 462 (Accession # Q02846-1).

Predicted N-terminus: Ala 52

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 46.7 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Biotinylation）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

GUCY2D，也称为CORD6、GUC1A4、GUC2D ROS-GC和RETGC-1，属于腺苷酸环化酶4类/鸟苷酸环化蛋白家族。在光感受器的视杆细胞和视锥细胞中催化环GMP（cGMP）的合成。通过调节cGMP的补充，在光传导中起着至关重要的作用。也可能参与将膜结合蛋白运输到感光器外段膜。