重组人Frizzled-2/FZD2蛋白-ACROBiosystems百普赛斯

Frizzled-2产品信息

英文名称：Frizzled-2

中文名称：卷曲蛋白-2

靶点别称：FZD2

物种：Human

标签：Fc Tag

属性：Protein

标记：Unconjugated

表达区间及表达系统（Source）

Human Frizzled-2, Fc Tag (FZ2-H5251) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Ser 156 (Accession # AAI13403).

Predicted N-terminus: Gln 24

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 41.7 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Frizzled-2（FZD2）也被称为FzE2，属于G蛋白偶联受体Fz/Smo家族。大多数卷曲受体与β-连环蛋白规范信号通路偶联，导致凌乱蛋白的激活、GSK-3激酶的抑制、β-连环素的核积累和Wnt靶基因的激活。FZD2包含一个FZ（卷曲）结构域。FZD2可能参与组织形态发生和/或分化组织中极性信息的转导和细胞间传递。FZD2的Lys-Thr-X-X-X-Trp基序与Dvl（发育不良）家族成员的PDZ域相互作用，并参与Wnt/β-catenin信号通路的激活。