重组人ApoA1蛋白-ACROBiosystems百普赛斯

Apo-A1产品信息

英文名称：Apolipoprotein A-I

中文名称：载脂蛋白A-I

靶点别称：APOA，Apo-A1

物种：Human

标签：His Tag

属性：Protein

标记：Unconjugated

表达区间及表达系统（Source）

Human Apo-A1, His Tag (AP1-H5225) is expressed from human 293 cells (HEK293). It contains AA Arg 19 - Gln 267 (Accession # NP_000030.1).

Predicted N-terminus: Arg 19

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 29.8 kDa. The protein migrates as 27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

载脂蛋白A1也称为载脂蛋白A-I、ApoA-I，是血浆中高密度脂蛋白（HDL）的主要蛋白质成分。它在脂质代谢中具有特殊作用。肠肠上皮细胞分泌的乳糜微粒也含有载脂蛋白A1，但它会在血液中迅速转移到高密度脂蛋白。这种蛋白质促进胆固醇从组织流出到肝脏排泄。它是卵磷脂胆固醇酰基转移酶（LCAT）的辅因子，负责大多数血浆胆固醇酯的形成。ApoA-I也被分离为前列环素（PGI2）稳定因子，因此可能具有抗凝血作用。编码它的基因缺陷与高密度脂蛋白缺乏症有关，包括丹吉尔病和系统性非神经性淀粉样变。此外，已经表明ApoA1通过与脂多糖或内毒素的相互作用参与HDL的抗内毒素功能。