重组人CD109蛋白-ACROBiosystems百普赛斯

CD109产品信息

英文名称：DNA polymerase alpha subunit

中文名称：DNA聚合酶α亚基

靶点别称：CD109,CPAMD7

物种：Human

标签：His Tag & Avi Tag

属性：Protein

标记：Biotin-labeled

表达区间及表达系统（Source）

Biotinylated Human CD109, His,Avitag (CD9-H82E4) is expressed from human 293 cells (HEK293). It contains AA Val 22 - Ser 1269 (Accession # Q6YHK3-1).

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 143.5 kDa. The protein migrates as 150-170 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

CD109是一种糖基磷脂酰肌醇（GPI）连接的糖蛋白，定位于血小板、活化T细胞和内皮细胞的表面。CD109在许多恶性肿瘤中表达，包括各种鳞状细胞癌和腺癌，并作为多功能辅助受体发挥作用。据报道，CD109与转化生长因子（TGF）-β受体相关，并负调控角质形成细胞中的TGF-β信号传导。此外，CD109可能与信号转导子和转录激活子-3信号传导和异常细胞增殖有关。