Cas12a核酸酶-ACROBiosystems百普赛斯

GENPower™ NLS-Cas12a Nuclease (MALS verified)

优势特色（Features）

With two NLS, which leads to transport to the nucleus efficiently.

High cleavage activity

产品描述（Product Details）

GENPower™ NLS-Cas12a Nuclease is a recombinant Lachnospiraceae bacterium Cas12 protein purified from Escherichia coli for CRISPR-based genome editing. The introduction of nuclear localization signals (NLS) can help Cas12 enter the nucleus, increasing the chance of genomic DNA cleavage.

应用说明（Application）

Developed as a variety of genome editing tools, such as transcription regulation, gene recombination, and base editing.

浓度（Concentration）

10 μg/μL

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

无菌（Sterility）

Negative

内毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method.

制剂（Formulation）

Supplied as 0.2 μm filtered solution in 20 mM NaAC, 500 mM NaCl, pH6.0 with glycerol as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

This product is stable after storage at:

The product MUST be stored at -20°C or lower upon receipt;

-20°C for 3 months under sterile conditions.

Cas12a分子背景

来自Lachnospiraceae细菌的簇状规则间隔短回文重复序列（CRISPR）相关内切酶Cas12a，也称为Cpf1，是一种1200-1500个氨基酸长的单体蛋白，属于CRISPR/Cas系统，这是一种原核生物的适应性免疫系统，现已成为基因组编辑的强大工具。CRISPR/Cas12a属于CRISPR/Cass系统的II类（5型），由单个亚基效应器定义。Cas12a最近成为Cas9的替代品，因为它具有独特的特征，如靶向富T基序的能力、不需要反式激活crRNA、诱导交错双链断裂以及RNA加工和DNA核酸酶活性的潜力。此外，Cas12a能够将结构更复杂的CRISPR/RNA（crRNA）前分子加工成成熟的crRNA，这使得将成熟或前crRNA用于基因组编辑成为可能。所有这些特征使CRISPR/Cas12a系统成为一种有价值的基因组工程工具。CRISPR-Cas12a已成功用于编辑许多哺乳动物细胞和植物的基因组。