重组人NOTCH3蛋白-ACROBiosystems百普赛斯

NOTCH3产品信息

英文名称：Neurogenic locus notch homolog protein 3

中文名称：神经源性基因座notch同源蛋白-3

靶点别称：NOTCH3

物种：Human

标签：Fc Tag

属性：Protein

标记：Unconjugated

表达区间及表达系统（Source）

Human NOTCH3, Fc Tag (NO3-H5255) is expressed from human 293 cells (HEK293). It contains AA Ala 40 - Glu 467 (Accession # Q9UM47-1).

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus

The protein has a calculated MW of 71.4 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Notch3是四种哺乳动物Notch蛋白之一，作为信号受体，参与发育模式、细胞命运决定、细胞存活和增殖的调节。Notch3可以与膜结合配体Jagged1、Jagged2和Delta1相互作用，调节细胞命运的决定。通过配体激活，缺口细胞内结构域（NICD）被释放并形成转录激活子RBPJ/RBPSHH复合物，激活分裂位点增强子的基因。因此，Notch3的激活可以导致分化、增殖和凋亡程序的实施。