重组人GMF-β蛋白-ACROBiosystems百普赛斯

Human GMF-beta Protein, His Tag (MALS verified)

分子别名（Synonym）

GMF-beta,Glia maturation factor beta,GMFB

表达区间及表达系统（Source）

Human GMF-beta, His Tag (GMA-H5145) is expressed from E. coli cells. It contains AA Ser 2 - His 142 (Accession # P60983).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 18.6 kDa. The protein migrates as 19-21 kDa under reducing (R) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative.

纯度（Purity）

>95% as determined by SDS-PAGE.

>98% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

胶质成熟因子β（GMF-β）是一种17 kDa的蛋白质神经生长因子，被鉴定为脊椎动物大脑中的生长和分化因子。

胶质成熟因子β刺激正常神经元和胶质细胞的分化。GMFB抑制N-18神经母细胞瘤系和C6胶质瘤系的增殖，同时促进其表型表达。

当加入细胞培养物中时，GMFβ会抑制胶质细胞和神经元的表型表达，从而抑制其各自肿瘤的增殖。尽管星形胶质细胞产生GMF-b并将其储存在细胞内，但它们不会将GMF-b分泌到培养基中。当细胞直接接触时，细胞表面GMFb在近距离内作用于靶细胞。GMF-β由胸腺上皮细胞产生，在T细胞发育中起着重要作用，有利于CD4+T细胞。

GMF-β是一种脑特异性蛋白，属于肌动蛋白结合蛋白（ADF）家族。GMF-β似乎在神经系统的分化、维持和再生中发挥作用。它还支持某些自身免疫性疾病的进展，可能是通过其诱导各种促炎细胞因子的产生和分泌的能力。