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  • 化合物 SPHINX31|T5194|TargetMol

化合物 SPHINX31|T5194|TargetMol

SPHINX31
1818389-84-2
￥319 1mg 起订
￥739 5mg 起订
￥996 10mg 起订
上海 更新日期：2024-09-30

TargetMol中国（陶术生物）

VIP3年
联系人：邵小姐
手机：4008200310 拨打
邮箱：marketing@tsbiochem.com

产品详情:

中文名称：
化合物 SPHINX31
英文名称：
SPHINX31
CAS号：
1818389-84-2
品牌：
TargetMol
产地：
美国
保存条件：
Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
纯度规格：
99.3%
产品类别：
抑制剂
货号：
T5194

Product Introduction

Bioactivity

NameSPHINX31
DescriptionSPHINX31 is a potent inhibitor of serine/arginine-rich protein kinase 1 (SRPK1; IC50: 5.9 nM).
Cell ResearchCells were transduced with gRNA vectors or treated with SPHINX31 and stained at the indicated time points with anti-mouse CD11b PE/Cy5 and anti-human CD11b PE or anti-human CD13 FITC. Data were analyzed by using LSRFortessa and FlowJo. Apoptosis levels were measured in human and/or mouse AML cells transduced with dual gRNA vectors (against SRPK1 and 3' BCL2 enhancer) and/or treated with 1 or 3?μM SPHINX31 at indicated time points, by using Annexin V. Data were analyzed by using LSRFortessa instruments. Cell cycle stages were measured in human and/or mouse AML cells transduced with dual gRNA vectors against SRPK1 and/or treated with 1 or 3?μM SPHINX31 at indicated time points, using Propidium Iodide. Data were analyzed using LSRFortessa instruments [2].
Animal ResearchFor in vivo experiments, 6–10-week-old female Rosa26Cas9/+ mice were treated triweekly for two weeks with either vehicle or 2?mg/kg SPHINX31. Four weeks post-treatment, bone marrow cells from these mice were freshly extracted (as mentioned above) and blocked with anti-mouse CD16/32 and 10% mouse serum. For the identification of LK/LSK, LT-HSC, myeloid and B-cell subpopulations, staining was performed using CD4 PE/Cy5, CD5 PE/Cy5, CD8a PE/Cy5, CD11b PE/Cy5, B220 PE/Cy5, TER-119 PE/Cy5, GR-1 PE/Cy5, SCA-1 Pacific Blue, CD150 PE/Cy7, CD34 FITC and CD117 APC-eFluor780. In each of the multi-colour flow cytometry experiments, we included the fluorescence minus one (FMO) controls. FMO controls provide a measure of spillover in a given channel. This allows for correct gating and selects only the stained cells in the experimental sample. Flow cytometry analysis was performed using a LSRFortessa instrument and resulting data were subsequently analyzed using FlowJo [2].
In vitroSPHINX31抑制了PC3细胞中丝/精氨酸富集剪接因子1（SRSF1）的磷酸化，该因子是SRPK1的底物（EC50：360nM），并在视网膜色素上皮（RPE）细胞中增加了抗血管生成VEGF-A165b剪接变体的表达[1]。对于MLL突变的AML细胞系，SPHINX31抑制生长，其IC50远低于其他AML细胞系，超过一个数量级。SPHINX31对正常小鼠造血干-祖细胞（HSPCs）的集落形成潜能没有影响。1.5、3和6μMSPHINX31并不影响正常人脐血CD34+细胞的集落形成能力[2]。
In vivo在体内，SPHINX31（每只眼2μg）抑制了小鼠脉络膜新生血管模型中的血管生长和巨噬细胞浸润[1]。向DBA2J小鼠注射0.8mg/kg SPHINX31（i.p.）后，24小时内血浆中的浓度达到0.225±0.036μM。在接受MOLM-13、THP-1细胞或第一次传导的患者源AMLs的异种移植RAIL小鼠中，SPHINX31（0.8或2.0 mg/kg）显著减少了白血病细胞的生长，并且随剂量增加显著延长了接受MOLM-13、THP-1和源于患者的MLL-X AMLs的小鼠的存活时间[2]。
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility InformationEthanol : 10 mg/mL
H2O : Insoluble
DMSO : 5.08 mg/mL (10 mM)
KeywordsSPHINX-31 | Serine-arginine protein kinases | retinal permeability | eye disease | neovascular | SPHINX 31 | Inhibitor | Norway Brown rats | SRPK | SPHINX31 | inhibit | Vascular endothelial growth factor receptor | HuCCA-1 | VEGFR | diabetics | angiogenic
Inhibitors RelatedSRPIN340 | SBC-115076 | Camostat mesylate | Fasudil hydrochloride | PCSK9-IN-10 | BMT-124110 Formate | FOY 251 | Cetraxate hydrochloride | Benzamidine hydrochloride | HA-1004 | CID755673 | Nafamostat mesylate
Related Compound LibrariesInhibitor Library | Bioactive Compound Library | Anti-Cancer Compound Library | Bioactive Compounds Library Max | Cell Cycle Compound Library | Metabolism Compound Library | Kinase Inhibitor Library | Apoptosis Compound Library | NO PAINS Compound Library | Target-Focused Phenotypic Screening Library
SPHINX31|TargetMol

公司简介

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。 凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

成立日期 (12年)
注册资本 566.265100万人民币
员工人数 100-500人
年营业额 ￥ 1亿以上
经营模式 贸易,工厂,试剂,定制,服务
主营行业 天然产物,生化试剂,分子生物学,分子砌块,生物技术服务

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