Polyscience 23966-2 线性聚乙烯亚胺（MW 25，000）

品牌故事——我的故事里怎能少了你？

Polyscience，Inc，成立于1961年，起家于化学但超越化学领域的美国制造商，总部位于宾夕法尼亚州沃灵顿，公司业务主要涵盖三大块：1.实验用产品：a）成千上万种具品牌特色的化合物和试剂，应用于实验室研究，组织学，电镜和解剖病理学以及其他诊断和生命科学研究；b）种类极其宽广的单体和聚合体，满足各方研究领域；c）有机/无机化合物，生物可降解材料，磁珠，荧光素，染料和特定抗体，以及常规蛋白包被颗粒（直径范围40nm~10mm）的全球首要制造工厂；d）顶端多聚化合物的供货中心，满足各种高性能工业领域需求，包括替代能源，汽车电子，国防&航空电子，便携式通信和计算机，医疗器械组装。2. 定制合成；3.cGMP生产服务。全球联系方式：1（800）523-2575；（215）343-6484。

Brand Story：

Me，Polyscience，Inc，Chemistry beyond the ordinary

Me，Polyscience，Inc，Headquartered at Warrington，PA，Call vial 1（800）523-2575

Me，Polyscience，Inc，Manufacturing hundreds of specialty chemicals and reagents for laboratory research, Histology, Light Microscopy and Anatomic Pathology as well as other clinical and life science applications.

Me，Polyscience，Inc，Stocking a wide portfolio of monomers and polymers for research applications.

Me，Polyscience，Inc，Developed to be the primary source for organic, inorganic, biodegradable, magnetic, fluorescent, dyed and specific antibody and general protein coated particles in diameters spanning the range of 40nm to 10mm.

Me，Polyscience，Inc，Offering innovative polymer products to meet the high performance requirements of a variety of industries including alternative energy, automotive electronics, defense & aerospace electronics, portable communications & computing, medical device assembly, and more. 

——Written/Edited by V. Shallan

Special Products：

你会喜欢的第二代聚合物转染试剂

Polyethylenimine，Linear，Mw 25，000 线性化聚乙烯亚胺（PEI 25，000）

基本描述：

线性化聚乙烯亚胺PEI 25，000，一种高电荷阳离子聚合物，非常容易结合于高电荷阴离子基质。工业应用上，线性化PEI可改善负电荷染料的物理外观，以调整染料的化学特性和增强染料与固相基质的黏附能力，常用作黏附增强剂，作用同多聚赖氨酸（poly-lysine）；科研应用上，线性化PEI能与DNA或其他负电荷生物大分子简单结合，正是这一特性，使得成为一种非常行之有效的载体运输介质（Vector carrier），即转染试剂。

化学性质：

订购信息：【原装进口，现货供应，产品有特价，欢迎咨询】

使用方法【来源于：CSH protocols，仅作交流学习】 ：

1. Pour ~450 mL of Milli-Q H₂O into a 500-mL glass beaker.

2. Add 500 mg of PEI to the beaker with stirring.

3. Add concentrated HCl drop-wise to the solution to pH <2.0.

4. Stir until the PEI is dissolved (~2-3 h). Maintain the pH <2.0 throughout.

Approximately 800 μL of 12 M HCl will be required for full PEI dissolution. There may still be some small fiber particles that will not dissolve.

5. Add concentrated NaOH drop-wise to the solution to pH 7.0.

Approximately 500 μL of 10 M NaOH will be required to neutralize the PEI solution.

6. Pour the solution into a 500-mL glass cylinder. Adjust the final volume to 500 mL with Milli-Q H₂O.

7. Filter-sterilize the solution through a 0.22-μm membrane.

8. Store aliquots of the desired volume at -20°C.

应用文献【基因转染用途】 ：

Zhong Z, Feijen J, Lok MC, Hennink WE, Christensen LV, Yockman JW, Kim YH, Kim SW. (2005). Low molecular weight linear polyethylenimine-b-poly(ethylene glycol)-b-polyethylenimine triblock copolymers: synthesis, characterization, and in vitro gene transfer properties.Biomacromolecules. 6(6):3440-8.

Sharon E. Reed, Elizabeth M. Staley, John P. Mayginnes, David J. Pintel (2006). Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors. Journal of Virological Methods. 138(1–2): 85–98.

Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy（2013）Transient Mammalian Cell Transfection with Polyethylenimine (PEI) . Methods Enzymol. 529: 227–240.

——Written/Edited by V. Shallan【版权归MKBio懋康所有】

更高效的PEI聚乙烯亚胺聚合物转染试剂

Polyethylenimine HCl MAX, Linear, Mw 40,000

线性化聚乙烯亚胺盐酸盐 MAX（PEI 40，000）

基本描述：

Polyethylenimine HCl MAX, Linear, Mw 40,000（PEI 40，000）是热卖产品线性化聚乙烯亚胺PEI25,000的升级产品，不同之处在于1）完全水解（去乙酰化）；2）PEI25，000的盐酸盐形式，具更高的水溶特性；3）含更长连续性乙烯亚胺（ethyleneimine）片段，其质子化氮水平比PEI25，000提高11%以上。本品经碱中和后，可转化为线性化自由胺（PEI），此时分子量正相当于25,000。

Thomas M，et al（2005）研究数据表明，去乙酰化的PEI 40,000（Polyethylenimine MAX 40,000）体外质粒DNA转染效率提高21倍，小鼠体内靶向效率达到10,000倍，随之肺部特异疗效显示增强1500倍。

化学性质：

订购信息：【原装进口，现货供应，产品有特价，欢迎咨询】

应用文献【基因转染用途】 ：

Mann JF, McKay PF, Arokiasamy S, Patel RK, Klein K, Shattock RJ. (2013). Pulmonary delivery of DNA vaccine constructs using deacylated PEI elicits immune responses and protects against viral challenge infection. J Control Release. 170(3):452-9.

Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A. 102(16):5679-84.

——Written/Edited by V. Shallan【版权归MKBio懋康所有】