化合物 AK-7|T5490|TargetMol

AK-7
420831-40-9

￥245 1mg 起订

￥349 2mg 起订

￥567 5mg 起订

上海更新日期：2024-09-30

TargetMol中国（陶术生物）

VIP3年

联系人：邵小姐

手机：4008200310 拨打

邮箱：marketing@tsbiochem.com

产品详情:

中文名称：: 化合物 AK-7

英文名称：: AK-7

CAS号：: 420831-40-9

品牌：: TargetMol

产地：: 美国

保存条件：: keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 98.43%

产品类别：: 抑制剂

货号：: T5490

Product Introduction

Bioactivity

名称	AK-7
描述	AK-7 is a brain-permeable SIRT2 inhibitor and to characterize its cholesterol-reducing properties in neuronal models with an IC50 of 15.5 μM.
细胞实验	Neuronal nuclear antigen (NeuN)-positive neurons and some astroglia were derived from mechanically dissociated ganglionic eminences of E16 rat embryos. The HD model based on the expression of mutant huntingtin has been described previously. Treatments of cultures with AK-7 were at 10 μM for 24 h unless stated otherwise. DMSO was included at the same concentrations as a control. Lower dose, chronic treatments with AK-7 were introduced to neurons at DIV4 and continued weekly coinciding with normal medium change [1].
激酶实验	Sirtuin activity was assessed using the Fleur de Lys assay with recombinant active enzymes SIRT1, SIRT2, and the catalytically active fragment of SIRT3. Results were measured using a Multilabel plate reader (excitation 355 nm, emission 460 nm). Assays were performed using the manufacturer's recommendations, and each compound concentration was tested in triplicate. For each experiment, SIRT1 activity was normalized to 1 U/reaction well and SIRT2 and SIRT3 activity to 5 U/reaction well (where U = 1 pmol/min at 37 °C, 250 μM substrate, 500 μM NAD+). Each reaction well-contained enzyme, 500 μM NAD+, 250 μM fluorogenic deacetylase substrate, supplied reaction buffer, and the compound of interest or mock control (DMSO) in a total volume of 50 μL. Autofluorescent backgrounds were measured in triplicate in reaction solutions containing substrate, buffer, and NAD+ in triplicate and subtracted from experimental signals [1].
动物实验	AK-7, solubilized at 1.5 mg/mL in 25% Cremophor EL (BASF)/ 10% DMSO in water, was administered by intraperitoneal injection to 11 week old mice at 15 mg/kg/dose. Blood was collected and centrifuged at 7,000 rpm for 7 min, and then serum was aspirated and immediately frozen in liquid nitrogen. Brains were immediately frozen in liquid nitrogen and stored at -80 C. Brains were weighed and then homogenized in four volumes of 10% Cremophor RH40 in water using homogenizer, and 2% v/v phosphoric acid was added to the homogenate, vortexed, and centrifuged at 10,000g at 25 C for 1 h. The supernatant was aspirated, and solid phase extraction was performed immediately. Serum samples were vortexed into 2% v/v phosphoric acid and centrifuged at 2500 rpm for 10 min[1]
体外活性	AK-7 is a selective cell- and brain-permeable SIRT2 inhibitor,this SIRT2 inhibitor stimulated cytoplasmic retention of sterol regulatory element binding protein-2 and subsequent transcriptional downregulation of cholesterol biosynthesis genes, resulting in reduced total cholesterol in primary striatal neurons. Furthermore, the identified inhibitor reduced cholesterol in cultured na?ve neuronal cells and brain slices from wild-type mice[1].AK-7 has roles in metabolic diseases, cancer, age-related disorders, and neurodegenerative diseases, potentially including Alzheimer's, Huntington's, and Parkinson's diseases[2][3].
体内活性	AK-7 (15 mg/kg/dose, i.p.) is brain-permeable in wild-type and HD mice[1].
存储条件	keep away from moisture,store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : 50 mg/mL (114.32 mM)
关键字	AK-7 \| Sirtuin \| AK7 \| AK 7 \| Inhibitor \| inhibit
相关产品	Fisetin \| Salvianolic acid B \| Nicotinamide riboside \| Nicotinamide \| Resveratrol \| Dihydrocoumarin \| Sirtinol \| JGB1741 \| SRT 2104 \| 3-TYP \| SIRT6-IN-5 \| MC3482
相关库	抑制剂库 \| 血脑屏障通透化合物库 \| 抗癌化合物库 \| 组蛋白修饰化合物库 \| 抗糖尿病库 \| 抗衰老化合物库 \| HIF-1化合物库 \| 谷氨酰胺代谢化合物库 \| 表观遗传库 \| 细胞重编程化合物库

AK-7|TargetMol

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

成立日期	(12年)
注册资本	566.265100万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,工厂,试剂,定制,服务
主营行业	天然产物,生化试剂,分子生物学,分子砌块,生物技术服务