化合物 (E)-Necrosulfonamide|T6904|TargetMol

Necrosulfonamide
1360614-48-7

￥198 1mg 起订

￥441 5mg 起订

￥579 10mg 起订

上海更新日期：2025-11-17

TargetMol中国（陶术生物）

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联系人：邵小姐

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邮箱：marketing@tsbiochem.com

产品详情:

中文名称：: 化合物 (E)-Necrosulfonamide

英文名称：: Necrosulfonamide

CAS号：: 1360614-48-7

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

纯度规格：: 99.49%

产品类别：: 抑制剂

货号：: T6904

Product Introduction

Bioactivity

名称	Necrosulfonamide
描述	Necrosulfonamide ((E)-Necrosulfonamide) is a necroptosis inhibitor that targets MLKL and is selective. Necrosulfonamide prevents the MLKL-RIP1-RIP3 necrosomal complex from interacting with its downstream effectors.
细胞实验	Necrosis inhibitors induce diverse effects on MLKL phosphorylation. HT-29 cells are treated with T/S/Z with or without necrosis inhibitors for 12 hr or 8 hr. The number of dead cells is determined by measuring released protease activity in culture medium. The whole-cell extracts are prepared and analyzed by western blotting. The final concentrations of 10 μM necrostatin-1 or 1 μM necrosulfonamide are used to block necrosis. (Only for Reference)
激酶实验	PRMT Biochemical Assays: A scintillation proximity assay (SPA) is used for assessing the effect of test compounds on inhibiting the methyl transfer reaction catalyzed by PRMTs. In brief, the tritiated S-adenosyl-L-methionine (3H-SAM) is used as the donor of methyl group. The (3H) methylated biotin labeled peptide is captured in a streptavidin/scintillant-coated microplate, which brings the incorporated 3H-methyl and the scintillant to close proximity resulting in light emission that is quantified by tracing the radioactivity signal (counts per minute) as measured by a TopCount NXT Microplate Scintillation and Luminescence Counter. When necessary, nontritiated SAM is used to supplement the reactions. The IC50 values are determined under balanced conditions at Km concentrations of both substrate and cofactor by titration of test compounds in the reaction mixture.
体外活性	方法：标记 Flag 的 RIP3-HT-29 细胞用Necrosulfonamide处理6小时，使用Flag标记的RIP3下拉实验检测RIP1和RIP3之间的相互作用。结果： Necrosulfonamide 作为 RIP3 下游坏死的抑制剂，并未阻断由坏死诱导的RIP1和RIP3之间的相互作用。[1]
存储条件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
溶解度	DMSO : 45 mg/mL (97.51 mM), Sonication is recommended.
关键字	substrate \| RIP3 \| Necrosulfonamide \| necrosome \| necrosis(MLKL) \| necrosis \| MLKs \| Mixed Lineage Kinase \| Inhibitor \| inhibit \| formation \| complex
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相关库	抑制剂库 \| 细胞焦亡化合物库 \| 经典已知活性库 \| 抗癌化合物库 \| 已知活性化合物库 \| 激酶抑制剂库 \| 抗纤维化化合物库 \| 细胞凋亡化合物库 \| HIF-1化合物库 \| NO PAINS 化合物库 \| 免疫/炎症分子化合物库 \| 疼痛相关化合物库

(E)-Necrosulfonamide|TargetMol

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

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