化合物 APS-2-79|T6760|TargetMol

APS-2-79 hydrochloride
2002381-31-7

￥152 1mg 起订

￥383 5mg 起订

￥618 10mg 起订

上海更新日期：2024-09-29

TargetMol中国（陶术生物）

VIP3年

联系人：邵小姐

手机：4008200310 拨打

邮箱：marketing@tsbiochem.com

产品详情:

中文名称：: 化合物 APS-2-79

英文名称：: APS-2-79 hydrochloride

CAS号：: 2002381-31-7

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 98.64%

产品类别：: 抑制剂

货号：: T6760

Product Introduction

Bioactivity

名称	APS-2-79 hydrochloride
描述	APS-2-79 hydrochloride (APS-2-79 HCl) is a MAPK antagonist that modulating KSR-dependent MAPK signalling by antagonizing RAF heterodimerization as well as the conformational changes required for phosphorylation and activation of KSR-bound MEK.
细胞实验	Cell viability assays are performed in 96 well plates. Optimal cell densities for 96 well plate assays are determined to obtain linear growth over the timecourse of assays. Specifically, A549, HCT-116, A375, SK-MEL-239, COLO-205, LOVO, SK-MEL-2, CALU-6, MEWO, SW620 and SW1417 cells are plated at 500 cells per well and treated with inhibitors for 72hrs before measuring viability. H2087 and HEPG2 cells are plated at 2000 cells per well, and treated with inhibitors for 72hrs. Cell viability is measured using resazurin, and the percent cell viability is determined by normalizing inhibitor-treated samples to DMSO controls(Only for Reference)
激酶实验	JNK phosphorylation: 500,000 cells/well are seeded in 6-well plates and incubated overnight. Cells are then incubated for 1 h with test compounds or DMSO as vehicle control (?nal concentration 1% v/v). Puromycin is added (?nal concentration of 18 μM) and cells incubated for a further 10 min to label nascent polypeptide chains. Background labelling is determined by incubating cells without puromycin. Cells are then washed in HBSS, harvested by scraping and centrifuged (300 g, 5 min). Cells are resuspended in 0.5 mL 50 mM DTT containing phosphatase inhibitors and incubated at 95℃ for 10 min. Samples are then snap frozen in liquid nitrogen and stored at -20℃ until blotted. Samples (20–30 μg protein/sample) are blotted onto a PVDF membrane. The membrane is blocked and incubated with anti-phospho-Thr183/Tyr185-JNK antibody overnight at 4℃. Secondary antibodies are used to label the primary antibody and detected using an infrared scanner. The intensity of the ?uorescence signal for anti-phospho-JNK antibody is background corrected and normalized for loading.
体外活性	APS-2-79通过直接结合KSR活性位点，作为RAF对MEK磷酸化的拮抗剂。在KSR缺失或使用KSR2(A690F)突变体进行体外实验时，APS-2-79失活。在Ras突变细胞系中，APS-2-79通过拮抗负反馈信号的释放，增强了几种MEK抑制剂的效能[1]。
存储条件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	H2O : < 1 mg/mL (insoluble or slightly soluble) DMSO : 90 mg/mL (212.3 mM) Ethanol : 30 mg/mL (70.8 mM)
关键字	MAP2K \| MAPKK \| Inhibitor \| Mitogen-activated protein kinase kinase \| APS 2 79 hydrochloride \| APS279 hydrochloride \| inhibit \| MEK \| APS-2-79 Hydrochloride
相关产品	Lidocaine hydrochloride \| sodium lauroyl-α-hydroxyethyl sulfonate \| PD 198306 \| Pelitinib \| Lidocaine \| Vemurafenib \| Honokiol \| Selumetinib \| NG25 \| Sodium lauryl sulfoacetate \| Trametinib \| SKLB-163
相关库	抑制剂库 \| 经典已知活性库 \| 已知活性化合物库 \| 抗胰腺癌化合物库 \| 激酶抑制剂库 \| MAPK 抑制剂库 \| 抗肥胖化合物库 \| 疼痛相关化合物库 \| 酪氨酸激酶分子库 \| 细胞重编程化合物库

APS-2-79 HCl|||APS-2-79|TargetMol

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

成立日期	(12年)
注册资本	566.265100万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,工厂,试剂,定制,服务
主营行业	天然产物,生化试剂,分子生物学,分子砌块,生物技术服务

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