化合物 MI-2|T2649|TargetMol

Menin-MLL inhibitor MI-2
1271738-62-5

￥248 1mg 起订

￥579 5mg 起订

￥828 10mg 起订

上海更新日期：2024-12-12

TargetMol中国（陶术生物）

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产品详情:

中文名称：: 化合物 MI-2

英文名称：: Menin-MLL inhibitor MI-2

CAS号：: 1271738-62-5

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 97.46%

产品类别：: 抑制剂

货号：: T2649

Product Introduction

Bioactivity

名称	Menin-MLL inhibitor MI-2
描述	Menin-MLL inhibitor MI-2 (MI2) is a potent menin-MLL interaction inhibitor with IC50 of 446 nM.
细胞实验	The MLL-AF9 and E2A-HLF transduced murine BMC are plated in 12-well plates at the concentration of 5×103 cells/mL with 1 mL methylcellulose medium M3234 containing 20% IMDM medium, 1% penicillin/streptomycin, IL-3 and 0.25% DMSO or compounds. 6 days later colonies are stained with 100 μL iodonitrotetrazolium chloride at final concentration of 1 mg/mL, incubated at 37°C for 30 min and counted. To replate for the 2nd round, colonies are counted at day 6 without staining and cells were washed out by 1×PBS buffer and resuspended in IMDM medium containing 15% FBS, 1% penicillin/streptomycin and IL-3. 5×103 cells are plated in 12-well plates with 1ml methycellulose medium M3234 containing 20% IMDM medium, 1% penicillin/streptomycin, IL-3 and 0.25% DMSO or compounds. 6 days later colonies are stained and counted.(Only for Reference)
激酶实验	High Throughput Screening: FITC-MBM1 at 15 nM and menin at 150 nM in the FP buffer are mixed and incubated for 1h in the dark at room temperature. For point screening, the 0.2 μL of each compound (20 μM final concentration, 1% DMSO) is added to 20 μL of the aliquot of the protein-peptide mixture and incubated on 384-well plates in the dark at room temperature for 1h. In confirmation screening, the serial dilution plates with compounds in DMSO are prepared and used to titrate the menin-FITC-MBM1 complex. Change in fluorescence polarization is monitored at 525 nm after excitations at 495 nm using the PHERAstar microplate reader (BMG) and applied to determine IC50 values with the Origin 7.0 program.
体内活性	MI-2能有效阻断含有不同MLL易位的人MLL白血病细胞系的细胞增殖,并诱导细胞凋亡。在HEK293细胞中,MI-2进入蛋白质靶标并有效抑制menin-MLL-AF9相互作用。MI-2通过下调MLL融合蛋白致癌活性必需的靶点基因的表达,有效阻断MLL融合蛋白介导的白血病转化。
存储条件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : 70 mg/mL (186.4 mM) H2O : < 1 mg/mL (insoluble or slightly soluble) Ethanol : 70 mg/mL (186.4 mM)
关键字	Menin MLL inhibitor MI 2 \| Apoptosis \| Inhibitor \| Epigenetic Reader Domain \| inhibit \| Menin-MLL inhibitor MI-2 \| MeninMLL inhibitor MI2
相关产品	L-Glutamic acid \| Metronidazole \| 5-Fluorouracil \| Curcumin \| Dextran sulfate sodium salt (MW 4500-5500) \| Stavudine \| Tributyrin \| Myricetin \| L-Ascorbic acid \| Acetylcysteine \| Salicylic acid \| Sodium 4-phenylbutyrate
相关库	抑制剂库 \| 经典已知活性库 \| 已知活性化合物库 \| 组蛋白修饰化合物库 \| 细胞凋亡化合物库 \| 抗衰老化合物库 \| NO PAINS 化合物库 \| 表观遗传库 \| PPI抑制剂库 \| 细胞重编程化合物库

MI-2|||MI2|||MI 2|||Menin-MLL inhibitor 2|||Menin-MLL Inhibitor|TargetMol

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

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