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网站主页 化工产品目录 生物化工 抑制剂 表观遗传学(Epigenetics) Histone Methyltransferase 抑制剂 MI-3 (Menin-MLL Inhibitor) 化合物 MI-3
  • 化合物 MI-3|T2643|TargetMol

化合物 MI-3|T2643|TargetMol

MI-3
1271738-59-0
397 1mg 起订
989 5mg 起订
1480 10mg 起订
上海 更新日期:2024-12-12

TargetMol中国(陶术生物)

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联系人:邵小姐
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邮箱:marketing@tsbiochem.com

产品详情:

中文名称:
化合物 MI-3
英文名称:
MI-3
CAS号:
1271738-59-0
品牌:
TargetMol
产地:
美国
保存条件:
store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
纯度规格:
98.97%
产品类别:
抑制剂
货号:
T2643

Product Introduction

Bioactivity

名称MI-3
描述MI-3 (Menin-MLL Inhibitor) (Menin-MLL Inhibitor) is an effective inhibitor of Menin-MLL interaction (IC50: 648 nM).
细胞实验The MLL-AF9 and E2A-HLF transduced murine BMC are plated in 12-well plates at the concentration of 5×103 cells/mL with 1 mL methylcellulose medium M3234 containing 20% IMDM medium, 1% penicillin/streptomycin, IL-3 and 0.25% DMSO or compounds. 6 days later colonies are stained with 100 μL iodonitrotetrazolium chloride at final concentration of 1 mg/mL, incubated at 37°C for 30 min and counted. To replate for the 2nd round, colonies are counted at day 6 without staining and cells were washed out by 1×PBS buffer and resuspended in IMDM medium containing 15% FBS, 1% penicillin/streptomycin and IL-3. 5×103 cells are plated in 12-well plates with 1ml methylcellulose medium M3234 containing 20% IMDM medium, 1% penicillin/streptomycin, IL-3 and 0.25% DMSO or compounds. 6 days later colonies are stained and counted. (Only for Reference)
激酶实验High Throughput Screening: FITC-MBM1 at 15 nM and menin at 150 nM in the FP buffer are mixed and incubated for 1h in the dark at room temperature. For point screening, the 0.2 μL of each compound (20 μM final concentration, 1% DMSO) is added to 20 μL of the aliquot of the protein-peptide mixture and incubated on 384-well plates in the dark at room temperature for 1h. In confirmation screening, the serial dilution plates with compounds in DMSO are prepared and used to titrate the menin-FITC-MBM1 complex. Change in fluorescence polarization is monitored at 525 nm after excitations at 495 nm using the PHERAstar microplate reader (BMG) and applied to determine IC50 values with the Origin 7.0 program.
体内活性在HEK293细胞中,MI-3与蛋白质靶点接近,进而对menin-MLL-AF9相互作用产生明显的抑制效果。在携带不同MLL易位的人MLL白血病细胞系中,MI-3有效阻断细胞分化并促使细胞凋亡。通过对MLL融合蛋白致癌活性所的靶点基因表达的下调,MI-3对MLL融合蛋白介导的白血病转化产生有效阻断作用。
存储条件store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度Ethanol : 16 mg/mL (42.6 mM)
H2O : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 16 mg/mL (42.6 mM)
关键字Apoptosis | MI3 | MI-3 | inhibit | Epigenetic Reader Domain | Inhibitor
相关产品L-Glutamic acid | Metronidazole | 5-Fluorouracil | Curcumin | Dextran sulfate sodium salt (MW 4500-5500) | Stavudine | Tributyrin | Myricetin | L-Ascorbic acid | Acetylcysteine | Salicylic acid | Sodium 4-phenylbutyrate
相关库抑制剂库 | 经典已知活性库 | 已知活性化合物库 | 组蛋白修饰化合物库 | 染色质修饰分子库 | 细胞凋亡化合物库 | 表观遗传库 | NO PAINS 化合物库 | PPI抑制剂库
MI 3|||Menin-MLL inhibitor 3|||Menin-MLL Inhibitor|TargetMol

公司简介

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿,致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区,已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库,以及多种类型的生化试剂产品,包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等,此外,我们还建设有CADD(计算机辅助药物设计)研究中心、药理实验室、药化合成平台三大技术中心,全方位满足客户的定制需求。 凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持,我们将有效帮助您缩短研发周期,取得更成功的结果。

成立日期 (12年)
注册资本 566.265100万人民币
员工人数 100-500人
年营业额 ¥ 1亿以上
经营模式 贸易,工厂,试剂,定制,服务
主营行业 天然产物,生化试剂,分子生物学,分子砌块,生物技术服务

化合物 MI-3相关厂家报价 更多

  • MI-3
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  • 深圳盛大医药科技有限公司
  • 2023-03-15
  • 询价
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