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网站主页 化工产品目录 生物化工 抑制剂 表观遗传学(Epigenetics) Histone Methyltransferase 抑制剂 UNC 0631 化合物 UNC0631
  • 化合物 UNC0631|T2354|TargetMol

化合物 UNC0631|T2354|TargetMol

UNC 0631
1320288-19-4
￥247 1mg 起订
￥619 5mg 起订
￥1160 10mg 起订
上海 更新日期：2024-12-12

TargetMol中国（陶术生物）

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联系人：邵小姐
手机：4008200310 拨打
邮箱：marketing@tsbiochem.com

产品详情:

中文名称：
化合物 UNC0631
英文名称：
UNC 0631
CAS号：
1320288-19-4
品牌：
TargetMol
产地：
美国
保存条件：
Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
纯度规格：
98.76%
产品类别：
抑制剂
货号：
T2354

Product Introduction

Bioactivity

名称UNC 0631
描述UNC 0631 is a effectvie histone methyltransferase G9a inhibitor (IC50=4 nM).
细胞实验MDA-MB-231, PC3, HCT116 cells are cultured in RPMI with 10% FBS, 22RV1 cells in alphaMEM and 10% FBS, MCF7 and IMR90 cells in DMEM with 10% FBS. Cells are treated with inhibitors for 48 h. The media is removed and replaced with DMEM 10% FBS without phenol red supplemented with 1 mg/mL of MTT and incubated for 1–2 h. Live cells reduce yellow MTT to purple formazan. The resulting formazanis solubilized in acidified isopropanol and 1% Triton. Formazan signal absorbance is measured at 570 nm and corrected for the 650 nm background. IC50s are calculated using GraphPad Prizm statistical package with sigmoidal variable slope dose response curve fit.(Only for Reference)
激酶实验SAHH-coupled assays: This assay utilizes SAHH to hydrolyze the methyltransfer product SAH to homocysteine and adenosine in the presence of adenosine deaminase which converts adenosine to inosine. The homocysteine concentration is then determined through conjugation of its free sulfhydryl moiety to a thiol-sensitive fluorophore, ThioGlo. For IC50 determinations, assay mixtures are prepared in 25 mM potassium phosphate buffer pH 7.5, 1 mM EDTA, 2 mM MgCl2, 0.01% Triton X-100 with 5 μM SAHH, 0.3 U/mL of adenosine deaminase, 25 μM SAM, and 15 μM ThioGlo. G9a, GLP, SETD7, SETD8, PRMT3 and SUV39H2 are assayed at 25 nM, 100 nM, 200 nM, 250 nM, 500 nM and 100 nM, respectively. Inhibitors are added at concentrations ranging from 4 nM to 16 μM. After 2 min incubation, reactions are initiated by addition of the histone peptides: 10 μM H3(1–25) for G9a, 20 μM H3(1–25) for GLP, 100 μM H3(1–25) for SETD7, 500 μM H4(1–24) for SETD8, 10 μM H4(1–24) for PRMT3 and 200 μM H3K9Me1 (1–15) for SUV39H2. The methylation reaction is followed by monitoring the increase in fluorescence using Biotek Synergy2 plate reader with 360/40 nm excitation filter and 528/20 nm emission filter for 20 min in 384 well-plate format. Activity values are corrected by subtracting background caused by the peptide or the protein. IC50 values are calculated using Sigmaplot. Standard deviations are calculated from two independent experiments.
体内活性在MCF7,22RV1和IMR90细胞中,UNC0631显著降低H3K9me2水平。
存储条件Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度Ethanol : 5 mg/mL (7.86 mM), Heating is recommended.
H2O : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 100 mg/mL (157.25 mM), Heating is recommended.
关键字H3K9me2 | p53 | UNC-0631 | chromatin | inhibit | EHMT1 | G9a | KMT1D | cell-penetration | PKMT | Inhibitor | Histone Methyltransferase | UNC 0631
相关产品Tazemetostat hydrobromide | XY1 | MRTX-1719 | MAK683 | GSK126 | Tazemetostat | iso-Azalansta | EPZ015666 | AMI-1 free acid | MS37452 | MAK-683 hydrochloride | Piribedil
相关库抑制剂库 | 经典已知活性库 | 已知活性化合物库 | 染色质修饰分子库 | 组蛋白修饰化合物库 | 表观遗传库
UNC0631|TargetMol

公司简介

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。 凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

成立日期 (12年)
注册资本 566.265100万人民币
员工人数 100-500人
年营业额 ￥ 1亿以上
经营模式 贸易,工厂,试剂,定制,服务
主营行业 天然产物,生化试剂,分子生物学,分子砌块,生物技术服务

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