化合物 Ciliobrevin A|T3156|TargetMol

Ciliobrevin A
302803-72-1

￥279 2mg 起订

￥438 5mg 起订

￥591 10mg 起订

上海更新日期：2024-09-29

TargetMol中国（陶术生物）

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产品详情:

中文名称：: 化合物 Ciliobrevin A

英文名称：: Ciliobrevin A

CAS号：: 302803-72-1

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.22%

产品类别：: 抑制剂

货号：: T3156

Product Introduction

Bioactivity

名称	Ciliobrevin A
描述	Ciliobrevin A (HPI-4) is an inhibitor of hedgehog signaling pathway with an IC50 <10 μM.
细胞实验	Using MTS-8 assay to measure cell proliferation and the toxicity of this drug. 2000 cells were plated in 96-well plates per well. HPI-4 was added to cells at concentrations of 0, 5 and 10 μM in 100 μl DMEM/F12 with 10% FBS and incubated for 0, 1, 3, 6 and 9 days. Then, 10 μl MST-8 was added to the media in each well and incubated in an environment without light for 90 min. The absorbance value was measured using an enzyme microplate reader at 450 nm wavelength. The relative viability of cells was expressed by OD value.(Only for Reference)
激酶实验	Smo-binding assays are conducted with BODIPY-cyclopamine and Smo-overexpressing HEK 293T cells, using a CMVpromoter-based SV40 origin-containing expression construct for Smo-Myc3 (murine Smo containing three consecutive Myc epitopes at the C terminus). HEK 293T cells are seeded into eight-well chambered coverslips (80,000 cells/well) and cultured in DMEM containing 10% FBS, 100 U/mL penicillin, and 0.1 mg/mL streptomycin. The cells are cultured until they reached 55 to 65% confluency (14-18 h), after which they are transfected with the Smo-Myc3 expression construct and Transit-LT1. Twenty-four hours after transfection, the cells are washed with PBS and cultured in DMEM containing 0.5% FBS, 5 nM BODIPY-cyclopamine, and various concentrations of either cyclopamine or individual HPIs. After 30 min, 10 μM Hoescht 33342 is added to each well, and the HPIs are incubated with the cells for an additional 30 min. The cells are then washed two times with PBS buffer, once with phenol red-free DMEM containing 0.5% FBS, and immediately imaged using a DMI6000B compound microscope. Images are background-substracted using ImageJ software with a rolling ball size of 75 pixels, and BODIPY-cyclopamine intensity is then determined using Metamorph software. Circular regions with a diameter of 300 pixels are placed over regions containing uniformly confluent cells, and the pixel intensities of approximately 20 regions from four independent images is used to determine the average BODIPY-cyclopamine levels for each experimental condition[1].
体外活性	经Ciliobrevin A培养的Shh-EGFPFLAG-Gli2细胞表现出原生纤毛的缩短，并且在一大部分经Ciliobrevin A处理的细胞中，该细胞器缺失。Ciliobrevin A扰乱Shh-LIGHT2FLAG-Gli1细胞中原生纤毛的形成，并促进FLAG-Gli1在此细胞器远端积累。Ciliobrevin A显著抑制这些神经前体细胞的增殖，通过组蛋白H3磷酸化水平(pH3)测量，并减少细胞内cyclin D1蛋白以及Gli1、Gli2和N-Myc转录本的水平。Ciliobrevin A能够阻止表达SmoM2的CGNPs的增殖，并应对缺乏Su(fu)功能的CGNPs同样有效，而Smo抑制剂Cyclopamine对这两种致癌损伤均无效。Ciliobrevin A阻止了Shh刺激下FLAG-Gli2全长/抑制剂比率的增加，但HPI-2和HPI-3无显著影响。Ciliobrevin A增加了纤毛中FLAG-Gli2的水平，这种增加与其对总FLAG-Gli2水平的影响不成比例[1]。
存储条件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : 65 mg/mL (181.47 mM), Sonication is recommended. Ethanol : 1 mg/mL (2.79 mM)
关键字	Hedgehog \| HPI 4 \| HPI4 \| inhibit \| Inhibitor \| Ciliobrevin A
相关产品	ALLO-2 \| Tolnaftate \| BMS-833923 \| SAG \| SMO-IN-2 \| Triparanol \| Ellagic acid \| Naftifine hydrochloride \| GANT 61 \| Jervine \| Vismodegib \| Itraconazole
相关库	抑制剂库 \| 经典已知活性库 \| 抗癌化合物库 \| 已知活性化合物库 \| 抗肥胖化合物库 \| GPCR靶点分子库 \| 膜蛋白靶向化合物库 \| Wnt/Hedgehog/Notch 通路化合物库 \| 神经元分化化合物库 \| 细胞重编程化合物库

HPI-4|||Hedgehog Pathway Inhibitor 4|TargetMol

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

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