小鼠Activin RIIA / ACVR2A蛋白-ACROBiosystems百普赛斯

Human Activin RIIA / ACVR2A Protein, Fc Tag

分子别名（Synonym）

Activin receptor type IIA,ACTRIIA

表达区间及表达系统（Source）

Human ACVR2A, Fc Tag (ACA-H5269) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 135 (Accession # P27037-1).

Predicted N-terminus: Pro

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 39.9 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

激活素受体2A型（ACVR2A）也称为激活素ⅡA型、ACTR-IIA、ACTRIIA和ACVR2，ACVR2是单程I型膜蛋白。ACVR2A属于蛋白激酶超家族、TKL-Ser/Thr蛋白激酶家族和TGFB受体亚家族。在配体结合上，ACVR2A可以形成由两种II型和两种I型跨膜丝氨酸/苏氨酸激酶组成的受体复合物。II型受体磷酸化并激活I型受体，I型受体自身磷酸化，然后结合并激活SMAD转录调节因子。ACVR2A也是激活素A、激活素B和抑制素A的受体。已经鉴定出几种I I型激活素受体，它们以不同的亲和力与不同的激活素结合。