环磷酰胺水合物|T0707|TargetMol

Cyclophosphamide hydrate
6055-19-2

￥135 5mg 起订

￥182 10mg 起订

￥276 25mg 起订

上海更新日期：2025-03-12

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产品详情:

中文名称：: 环磷酰胺水合物

英文名称：: Cyclophosphamide hydrate

CAS号：: 6055-19-2

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | Shipping with blue ice.

纯度规格：: ≥95%

产品类别：: 抑制剂

货号：: T0707

Product Introduction

Bioactivity

名称	Cyclophosphamide hydrate
描述	Cyclophosphamide hydrate is a DNA alkylating agent, an inhibitor of DNA synthesis. Cyclophosphamide hydrate has antitumor and immunosuppressive activities.
细胞实验	9L/pBabe, 9L/Bax, and 9L/Bcl-2 cells are treated with 12, 24, or 50 μM MFA for 72 h. Cells remaining on the plates at 0, 24, 48, and 72 h are washed twice with cold PBS and then stained for 5 min with crystal violet [1.25 g of crystal violet dissolved in a solution containing 50 mL of 37% formaldehyde and 450 mL of methanol]. The stained cells are washed three times in tap water and the plates are allowed to dry. The stain is eluted from the cells with 70% ethanol and the absorbance is then read at 595 nm. The staining intensity of each drug-treated sample (A?595) is then graphed as a percentage of the staining intensity at the 0-h time point.
激酶实验	9L cells are treated with drug for the times indicated in each experiment. Floating and attached cells are collected, pooled, resuspended in lysis buffer (10 mM HEPES buffer, pH 7.4, containing 2 mM EDTA, 0.1% CHAPS detergent, 5 mM DTT, 350 ng/mL phenylmethylsulfonyl fluoride, 10 ng/mL pepstatin A, 10 ng/mL aprotinin, and 20 ng/mL leupeptin) and lysed by three freeze-thaw cycles (alternating between a dry ice isopropanol bath and a 37°C water bath). Lysates are spun in a bench top centrifuge at full speed for 15 min and the supernatant (cell extract) fraction transferred to a new tube. Cell extracts (20 μL) are assayed for caspase 9, caspase 8, and caspase 3 activity by incubation at 37°C for either 1 h (caspase 3) or 3 h (caspase 9 and caspase 8) in 500 μL of reaction buffer (10 mM HEPES, pH 7.4, 2 mM EDTA, 0.1% CHAPS, and 5 mM DTT) containing 50 μM caspase form-selective substrate: Ac-LETD-AFC for caspase 8; Ac-LEHD-AFC for caspase 9; and Ac-DEVD-AMC for caspase 3. Background activity is determined for each sample as follows. Cell extracts are preincubated for 15 min at room temperature, with or without caspase form-selective inhibitor: 1 μM z-LETD-FMK for caspase 8, 1 μM z-LEHD-FMK for caspase 9, and 5 μL of Casputin for caspase 3. Caspase activity measured in the absence of inhibitor is divided by the background caspase activity measured in the presence of inhibitor. A value of 1 is subtracted from each measured activity, such that a caspase activity of 0 corresponds to no increase in the specific caspase activity with drug treatment. Fluorescence of the caspase product (excitation at 395 nm and emission at 525 nm for AFC substrates, and excitation at 380 nm and emission at 460 nm for the AMC substrate) is measured using a Shimadzu model RF-1501 spectrofluorophotometer and the manufacturer's PC-1501 software package.
体外活性	方法：巨噬细胞 Raw 264.7 用 Cyclophosphamide hydrate (10-250 μg/mL) 处理 48 h，使用 MTT assay 检测细胞活力。结果：Cyclophosphamide hydrate 对 Raw 264.7 有细胞毒性，IC50 为 145.44 μg/mL。[1] 方法：人乳腺癌细胞 MDA-MB-231 和 MDA-MB-435S 用 Cyclophosphamide hydrate (0.25-1 mM) 处理 4-24 h，使用 Wound healing assay 检测细胞迁移情况。结果：迁移的 MDA-MB-231 细胞数量的增加取决于 Cyclophosphamide 的浓度，而到 MDA-MB-435S 细胞的迁移显著减少，并且与 Cyclophosphamide 浓度无关。[2]
体内活性	方法：为检测体内抗肿瘤活性，将 Cyclophosphamide hydrate (50-150 mg/kg) 单次腹腔注射给携带小鼠结直肠肿瘤 CT26 的 BALB/c 小鼠。结果：Cyclophosphamide hydrate 诱导肿瘤体积显著减少。[3] 方法：为检测体内抗肿瘤活性，将 Cyclophosphamide hydrate (140 mg/kg) 口服给药给携带小鼠乳腺癌肿瘤 4T1 的 Balb/c 小鼠，每六天一次，持续十八天。结果：Cyclophosphamide hydrate 治疗显著抑制了小鼠的肿瘤生长。[4]
存储条件	Powder: -20°C for 3 years \| Shipping with blue ice.
溶解度	DMSO : 50 mg/mL (179.15 mM), The compound is unstable in solution. Please use soon. H2O : 7 mg/mL (25.08 mM) Ethanol : < 1 mg/mL (insoluble or slightly soluble)
关键字	DNA Alkylator/Crosslinker \| Cyclophosphamide Monohydrate \| inhibit \| Cyclophosphamide \| Cyclophosphamide hydrate \| Cyclophosphamide Hydrate \| Inhibitor
相关产品	Crystal Violet \| Benzocaine \| Flurbiprofen \| Cisplatin \| N-Nitroso-N-methylurea \| Clarithromycin \| Methoxsalen \| Temozolomide \| Busulfan \| Cholesterol \| Prednisone \| Carboplatin

环磷酰胺一水物|||环磷酰胺水合物|||Cyclophosphamide monohydrate|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子