新冠刺突蛋白Spike S1-ACROBiosystems百普赛斯

SARS-CoV-2 (COVID-19) S1 protein, His Tag (MALS verified)

分子别名（Synonym）

Spike,S1 protein,Spike glycoprotein Subunit1,S glycoprotein Subunit1,Spike protein S1

表达区间及表达系统（Source）

SARS-CoV-2 S1 protein, His Tag (S1N-C52H3) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1).

Predicted N-terminus: Val 16

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 76.9 kDa. The protein migrates as 100-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

冠状病毒可以通过与人ACE2受体相互作用感染人呼吸道上皮细胞。刺突蛋白是一种大的I型跨膜蛋白，含有两个亚基S1和S2。S1主要包含一个受体结合结构域（RBD），负责识别细胞表面受体。S2包含膜融合所需的基本元件。S蛋白在诱导中和抗体和T细胞反应以及保护性免疫中起着关键作用。