重组人VEGFR2蛋白-ACROBiosystems百普赛斯

Human VEGFR2 / KDR Protein, His Tag (MALS verified)

分子别名（Synonym）

KDR,CD309,FLK1,VEGFR,VEGFR2

表达区间及表达系统（Source）

Human VEGF R2, His Tag (KDR-H5227) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Glu 764 (Accession # AAI31823.1).

Predicted N-terminus: Ala 20

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 85.2 kDa. The protein migrates as 125-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

VEGFR2也称为CD309，是VEGFR的亚型之一。VEGF受体是血管内皮生长因子（VEGF）的受体。VEGFR有三种主要亚型，编号为1、2和3。VEGF受体具有由7个免疫球蛋白样结构域组成的细胞外部分、一个跨膜区和一个含有分裂酪氨酸激酶结构域的细胞内部分。VEGF-A与VEGFR-1（Flt-1）和VEGFR-2（KDR/Flk-1）结合。VEGFR-2似乎介导了几乎所有已知的细胞对VEGF的反应。VEGFR-1的功能不太明确，尽管它被认为可以调节VEGFR-2信号传导。VEGFR-1的另一个功能可能是充当假受体/诱饵受体，将VEGF与VEGFR-2结合隔离开来（这在胚胎血管生成过程中似乎尤为重要）。此外，VEGFR2能够在VEGF激活后与HIV-1细胞外Tat蛋白相互作用，似乎可以增强卡波济氏肉瘤病变中的血管生成。