氯硝柳胺|T0711|TargetMol

Niclosamide
50-65-7

￥320 500mg 起订

上海更新日期：2025-03-12

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产品详情:

中文名称：: 氯硝柳胺

英文名称：: Niclosamide

CAS号：: 50-65-7

品牌：: TargetMol

产地：: 美国

保存条件：: keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 96.83%

产品类别：: 抑制剂

货号：: T0711

Product Introduction

Bioactivity

名称	Niclosamide
描述	Niclosamide (Niclocide)(BAY2353) is used to treat most tapeworm infections by inhibiting DNA replication.
细胞实验	Cells are plated in 96-well culture plates with cell density of 3-4 × 103 cells/well and treat with Niclosamide by adding 100 μL medium containing Niclosamide of various concentrations on the second day. After 72-hour's treatment, MTT is added to each well and incubated for additional 4-5 hours, and the absorbance is measured on a microplate reader at 570 nM. Cell growth inhibition is evaluated as the ratio of the absorbance of the sample to that of the control. The results are representative of at least 3 independent experiments. (Only for Reference)
激酶实验	Protein Kinase profiling assay: Assay for 22 different proteins kinases is carried out by ProQinase Gmbh. All of the protein kinases are expressed either in Sf9 insect cells or in E.coli as recombinant GST-fusion proteins or His-tagged proteins. Protein kinases are purified by affinity chromatography using either GSH-agarose or Ni_NTH-agarose. A radiometric protein kinase assay is used for measuring the kinase activity of the 22 protein kinases. Briefly, for each protein kinase, 50 μL reaction cocktail containing 60 mM HEPES-NaOH, 3 mM MgCl2, 3 mM MnCl2, 3 μM Na-orthovanadate, 1.2 mM DTT, 50 μg/mL PEG20000, 1 μM [γ-33P]-ATP, Niclosamide, adequate amount of enzyme and its substrate. The PKC-alpha assay additionally contain 1 mM Cacl2, 4 mM EDTA, 5 μg/mL phosphatidylserine and 1 μg/mL 1, 2-Dioleyl-glycerol. The reaction cocktails are incubated at 37 °C for 60 minutes and stop with 50 μL 2% (v/v) H3PO4. Incorporation of 33Pi is determined with a microplate scintillation counter. The activities and the IC50 values are calculated using Quattro Workflow V2.28.
体外活性	Niclosamide (< 5 μM) 在HeLa细胞中剂量依赖性抑制STAT3介导的荧光素酶报告活性，其IC50为0.25 μM。Niclosamide (< 2 μM) 在Du145细胞中剂量依赖性抑制STAT3的磷酸化。Niclosamide (1 μM) 抑制在Du145细胞中EGF诱导的STAT3核内转移。Niclosamide (< 2 μM) 在Du145细胞中剂量依赖性抑制STAT3下游基因的转录。Niclosamide (< 10 μM) 在Du145癌细胞中剂量依赖性诱导G0/G1停滞和凋亡。[1] Niclosamide能够在感染SARS-CoV的Vero E6细胞中以微摩尔浓度抑制SARS-CoV的复制。[2] Niclosamide (< 7.5 μM) 促进Frizzled1的内吞作用，下调Dishevelled-2蛋白，并在U2OS细胞中抑制Wnt3A刺激的beta-catenin稳定性和LEF/TCF报告活性。[3] Niclosamide在U2OS细胞中剂量和时间依赖性抑制TNF诱导的NF-κB报告活性。Niclosamide (125 nM) 抑制由p65、IKKα、IKKβ、IKKγ和TAK1在U2OS细胞中诱发的NF-κB激活。Niclosamide (< 500 nM) 完全阻断在HL-60细胞中TNFα诱导的NF-κB–DNA复合体的时间和剂量依赖性变化。Niclosamide (< 10 nM) 抑制U266细胞中固有的NF-κB激活。Niclosamide在HL-60、Molm13或AML原发细胞中剂量和时间依赖性抑制TNF诱导的IκBα降解和p65重新定位。Niclosamide (500 nM) 降低 TNF诱导的与HL-60细胞生存相关的NF-κB依赖性基因产品。Niclosamide剂量依赖性抑制AML细胞的生长并有效诱发凋亡，降低Mcl-1和XIAP水平，增加细胞内ROS水平。[4]
体内活性	Niclosamide（每日每公斤40毫克，腹腔注射）抑制裸鼠体内荷HL-60异种移植瘤的AML细胞生长。[4]
存储条件	keep away from moisture \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	10% DMSO+40% PEG300+5% Tween 80+45% Saline : 0.63 mg/mL (1.93 mM), Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. DMSO : 6.25 mg/mL (19.11 mM)
关键字	SW-13 \| antihelminthic \| BAY-2353 \| inhibit \| BD140A \| Parasite \| Antibiotic \| BAY 2353 \| STAT \| NCI-H295R \| Vero E6 \| Hela \| Niclosamide \| Inhibitor \| bayluscide
相关产品	G-418 disulfate \| Doxycycline \| Neomycin sulfate \| Metronidazole \| Hydroxychloroquine \| Nitazoxanide \| EDTA copper(II) disodium salt \| Ampicillin sodium \| Sulfamethoxazole sodium \| Kanamycin sulfate \| Kojic acid \| Kaempferol
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氯硝柳胺|||Niclocide|||BAY2353|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
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