单克隆Anti-G4S linker抗体

APC-Labeled Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) (0.03% Proclin)

抗体来源（Source）

Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) is a rabbit monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply. It shows superior performance in flow cytometry assays with higher affinity and better specificity.

应用（Application）

Flow Cytometry (Evaluation of cell surface expressed CARs of varying specificity containing a G4S linker within the scFv of the extracellular domain).

克隆号（Clone）

016

种属（Species）

Rabbit

亚型（Isotype）

Rabbit IgG | Rabbit Kappa

特异性（Specificity）

Specifically recognizes the scFv-based CARs containing a G4S linker.

偶联（Conjugate）

APC

Excitation Wavelength: 640 nm

Emission Wavelength: 661 nm

推荐稀释比（Recommended Dilution）

1:50

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 0.2% BSA, 0.03% Proclin300 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 24 months in lyophilized state;

-70°C for 12 months after reconstitution.

2-8 °C for 12 months after reconstitution.

背景（Background）

The G4S linker is emerged as the most common linking peptides for scFvs. It is composed of the repeated sequence (Gly-Gly-Gly-Gly-Ser) and commonly found as either a 15-mer (G4S)3 or 20-mer(G4S)4 within scFv fragments. The G4S linker has become an integral part of the vast majority of CARs as it links the VH and VL recognition domains of scFvs. The G4S linker is commonly used in CARs targeting solid tumor antigens, such as HER2 and CEA, while the whitlow/218 linker peptide is predominantly used in CAR constructs targeting hematological malignancies such as CD19 CAR products.