FITC-Labeled Cynomolgus CD20蛋白, His Tag

FITC-Labeled Cynomolgus CD20 / MS4A1 Full Length Protein, His Tag (Nanodisc)

分子别名（Synonym）

MS4A1,CD20,MS4A-1

表达区间及表达系统（Source）

FITC-Labeled Cynomolgus CD20 Full Length Protein, His Tag (CD0-CF2H3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Pro 297 (Accession # M4ZHZ6-1 ).

Predicted N-terminus: Met 1

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 35.2 kDa.

Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution.

The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein(MSP1D1) upon removal of the detergent.

偶联（Conjugate）

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记（Labeling）

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>85% as determined by SDS-PAGE.

制剂（Formulation）

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

The product MUST be stored at -70°C or lower upon receipt;

-70°C for 3 months under sterile conditions.

背景（Background）

B淋巴细胞抗原CD20也称为B淋巴细胞表面抗原B1、白细胞表面抗原Leu-16、跨膜4结构域亚家族A成员1和MS4A1，是一种活化的糖基化磷蛋白，从B前相（CD45R+、CD117+）开始在所有B细胞表面表达，浓度逐渐增加，直到成熟。CD20在B细胞发育的所有阶段都有表达，除了第一个和最后一个阶段；它从晚期pro-B细胞通过记忆细胞存在，但不存在于早期pro-B细胞或浆母细胞和浆细胞上。在B细胞淋巴瘤、毛细胞白血病、B细胞慢性淋巴细胞白血病和黑色素瘤癌症干细胞中发现。该蛋白没有已知的天然配体，其功能是实现最佳的B细胞免疫反应，特别是针对T非依赖性抗原。怀疑它在细胞膜中充当钙通道。CD20/MS4A1是单克隆抗体（mAb）利妥昔单抗、伊维单抗和托西莫单抗的靶点，这些抗体都是治疗所有B细胞淋巴瘤和白血病的活性药物。CD20/MS4A1的缺陷是免疫缺陷共同变量5型（CVID5）的原因；也称为由于CD20缺陷引起的抗体缺乏症。CVID5是一种原发性免疫缺陷，其特征是抗体缺乏、低丙种球蛋白血症、反复细菌感染和无法对抗原产生抗体反应。