小鼠Carbonic Anhydrase XII/CA XII蛋白-ACROBiosystems百普赛斯

Biotinylated Mouse Carbonic Anhydrase XII Protein, His,Avitag™ (active enzyme, MALS verified)

分子别名（Synonym）

Carbonic Anhydrase XII,CA XII,Car12,CA-XII,Carbonate dehydratase XII

表达区间及表达系统（Source）

Biotinylated Mouse Carbonic Anhydrase XII Protein, His,Avitag (CAI-M82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Ser 301 (Accession # Q8CI85-1).

Predicted N-terminus: Ala 25

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 35.0 kDa. The protein migrates as 42-47 kDa under reducing (R) condition (SDS-PAGE).

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, PH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

该基因编码α-碳酸酐酶家族的一个膜结合成员，该酶催化二氧化碳可逆水合为碳酸氢盐。这些蛋白质参与各种生物过程，包括呼吸、钙化、酸碱平衡、骨吸收以及房水、脑脊液、唾液和胃酸的形成。选择性剪接导致多种转录变体。