小鼠NKG2A蛋白-ACROBiosystems百普赛斯

Mouse NKG2A / CD159a Protein, His Tag

分子别名（Synonym）

NKG2A,CD159a,KLRC1,NK cell receptor A

表达区间及表达系统（Source）

Mouse NKG2A, His Tag (NKA-M52H5) is expressed from human 293 cells (HEK293). It contains AA Thr 95 - Ile 244 (Accession # Q9Z202-1).

Predicted N-terminus: Thr 95

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 19.1kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

NKG2A/CD159a是一种跨膜蛋白，属于CD94/NKG2家族的C型凝集素样受体，可抑制先天免疫系统激活，也称为KLRC1、CD159a、NK细胞受体a和NKG2-a/NKG2-B II型整合膜蛋白。NKG2A标记了一种独特的免疫效应子亚群，优先共表达组织驻留CD103分子，但不表达免疫检查点抑制剂。NKG2A阻断疗法通过CD8 T细胞而非NK细胞进行。NKG2A表达的增加可能是由IL-10诱导的，IL-10在HCC患者的血浆中高水平存在。阻断IL-10可以特异性抑制NK细胞中NKG2A的表达。这些发现表明，NKG2A的表达受癌症巢因子的影响，并导致NK细胞耗竭，这表明NKG2A阻断有可能通过逆转NK细胞耗竭来恢复对肝肿瘤的免疫力。