小鼠Claudin-18.2蛋白-ACROBiosystems百普赛斯

Mouse Claudin-18.2 Protein, His,Twin-Strep Tag (Detergent)

分子别名（Synonym）

Claudin-18.2,CLDN18,Claudin-18

表达区间及表达系统（Source）

Mouse Claudin-18.2, His,Twin-Strep Tag (CL2-M5585) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Thr 203 (Accession # P56857-3).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus and a twin strep tag at the C-terminus.

The protein has a calculated MW of 26.7 kDa. The protein migrates as 40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>80% as determined by SDS-PAGE.

制剂（Formulation）

This product is not suitable for cell based experiments due to cytotoxicity of DDM.

DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.

DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.

If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

The product MUST be stored at -70°C or lower upon receipt;

-70°C for 12 months under sterile conditions.

背景（Background）

Claudins（CLDN）是一个蛋白质家族，形成紧密连接并维持上皮细胞和内皮细胞的极性。CLDN18在胃和肺中特异性表达。在通过选择性剪接产生的两种CLDN18亚型转录物中，CLDN18.2是一种高度选择性的胃谱系标志物，可确定肿瘤状况下的胃表型，而CLDN18.1是肺特异性的。CLDN18.2是一种高度选择性的胃谱系抗原，仅在短暂分化的胃上皮细胞上表达，其对抗体药物的可及性有限。14，15 CLDN18.2在恶性转化过程中保持不变，因此经常显示在人类癌症细胞表面。