小鼠Cathepsin L/CTSL1蛋白-ACROBiosystems百普赛斯

Mouse Cathepsin L / CTSL1 Protein, His Tag (MALS verified)

分子别名（Synonym）

CTSL1,MEP,CATL,CTSL

表达区间及表达系统（Source）

Mouse Cathepsin L Protein, His Tag (CAL-M52H3) is expressed from human 293 cells (HEK293). It contains AA Thr 18 - Asn 334 (Accession # P06797-1).

Predicted N-terminus: Thr 18

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus. This protein contains an Activation peptide, and will be partially processed into Pro form with calculated MW of 35.8 kDa and proteolytic cleavage generate the single-chain activate form with calculated MW of 24.1 kDa and cleavaged activation peptide with calculated MW of 5 kDa under reducing (R) condition. The protein migrates as 40 kDa, 30 kDa and 10 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Supplied as 0.2 μm filtered solution in 25 mM Tris, 150 mM NaCl, pH7.0 with glycerol as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

组织蛋白酶L（CTSL1）也被称为主要排泄蛋白（MEP），是肽酶C1家族的成员，是由二硫键连接的重链和轻链组成的二聚体。CTSL1是一种溶酶体半胱氨酸蛋白酶，在细胞内蛋白质分解代谢中起着重要作用。其底物包括胶原蛋白和弹性蛋白，以及α-1蛋白酶抑制剂，这是中性粒细胞弹性蛋白酶活性的主要控制因素。MEP与多种病理过程有关，包括肌病和心肌缺血中的肌原纤维坏死，以及对蛋白尿的肾小管反应。CTSL1对溶酶体中蛋白质的整体降解很重要。MEP的特异性接近木瓜蛋白酶。与组织蛋白酶B相比，组织蛋白酶L对蛋白质底物表现出更高的活性，但对Z-Arg-Arg-NHMec几乎没有活性，也没有肽基二肽酶活性。在pH 4.5至6.5的弱酸性条件下，人组织蛋白酶L活性最大。酶的稳定性在较高的pH值下降低。